Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100687
Name   oriT_pS50-A in_silico
Organism   Escherichia coli strain S50
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP010239 (137532..138081 [+], 550 nt)
oriT length   550 nt
IRs (inverted repeats)      243..255, 257..269  (TATTTAATAATTC..GAATTATTAAATA)
 311..318, 321..328  (GCAAAAAC..GTTTTTGC)
Location of nic site      337..338
Conserved sequence flanking the
  nic site    		  GTAGTGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 550 nt

>oriT_pS50-A
TTACTCTGGCCATAAGATAAAACCTTTCATTATTAAGCAATGAACTTTTCACTATAAATATGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTATAAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAATTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTCTCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAAACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCGAAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGAGAGCGATATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   759 GenBank   WP_073569668
Name   TraI_pS50-A insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191573.10 Da        Isoelectric Point: 5.6836

>WP_073569668.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYMDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQTRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHTEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVMRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTQASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMQNGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQIERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1434-1557)

AAA domain (AAA_30)

(968-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 111916..138469

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG72_RS26110 (RG72_24710) 107591..107938 + 348 WP_000624622 IS66 family insertion sequence element accessory protein TnpB -
RG72_RS26115 (RG72_24715) 107958..109529 + 1572 WP_000381395 IS66-like element ISCro1 family transposase -
RG72_RS26120 (RG72_24720) 109695..110432 - 738 WP_073569706 hypothetical protein -
RG72_RS26125 (RG72_24725) 110635..111366 - 732 WP_000782451 conjugal transfer complement resistance protein TraT -
RG72_RS26130 (RG72_24730) 111415..111900 - 486 WP_000605870 hypothetical protein -
RG72_RS26135 (RG72_24735) 111916..114738 - 2823 WP_001007039 conjugal transfer mating-pair stabilization protein TraG traG
RG72_RS26140 (RG72_24740) 114735..116108 - 1374 WP_000944331 conjugal transfer pilus assembly protein TraH traH
RG72_RS26145 (RG72_24745) 116095..116487 - 393 WP_000660699 F-type conjugal transfer protein TrbF -
RG72_RS26150 (RG72_24750) 116468..116815 - 348 WP_001309242 P-type conjugative transfer protein TrbJ -
RG72_RS26155 (RG72_24755) 116745..117290 - 546 WP_000059831 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
RG72_RS26160 (RG72_24760) 117277..117561 - 285 WP_000624194 type-F conjugative transfer system pilin chaperone TraQ -
RG72_RS26165 (RG72_24765) 117680..118024 - 345 WP_000556796 conjugal transfer protein TrbA -
RG72_RS26170 (RG72_24770) 118038..118781 - 744 WP_001030371 type-F conjugative transfer system pilin assembly protein TraF traF
RG72_RS26175 (RG72_24775) 118774..119031 - 258 WP_000864353 conjugal transfer protein TrbE -
RG72_RS26180 (RG72_24780) 119058..120908 - 1851 WP_000821856 type-F conjugative transfer system mating-pair stabilization protein TraN traN
RG72_RS26185 (RG72_24785) 120905..121543 - 639 WP_001080256 type-F conjugative transfer system pilin assembly protein TrbC trbC
RG72_RS26190 (RG72_24790) 121552..121857 - 306 WP_000224416 hypothetical protein -
RG72_RS26195 (RG72_24795) 121887..122879 - 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
RG72_RS26200 (RG72_24800) 122876..123508 - 633 WP_001203728 type-F conjugative transfer system protein TraW traW
RG72_RS26205 (RG72_24805) 123505..123891 - 387 WP_023352028 type-F conjugative transfer system protein TrbI -
RG72_RS26210 (RG72_24810) 123888..126518 - 2631 WP_012917677 type IV secretion system protein TraC virb4
RG72_RS27935 (RG72_24815) 126644..126991 - 348 WP_000836682 hypothetical protein -
RG72_RS26220 (RG72_24820) 127019..127237 - 219 WP_000556745 hypothetical protein -
RG72_RS26225 (RG72_24825) 127317..127790 - 474 WP_000549568 hypothetical protein -
RG72_RS26230 (RG72_24830) 127783..128196 - 414 WP_000549589 hypothetical protein -
RG72_RS26235 (RG72_24835) 128189..128410 - 222 WP_001278683 conjugal transfer protein TraR -
RG72_RS26240 (RG72_24840) 128545..129060 - 516 WP_000809881 type IV conjugative transfer system lipoprotein TraV traV
RG72_RS26245 (RG72_24845) 129057..129377 - 321 WP_001057307 conjugal transfer protein TrbD -
RG72_RS26250 (RG72_24850) 129364..129930 - 567 WP_000896599 conjugal transfer pilus-stabilizing protein TraP -
RG72_RS26255 (RG72_24855) 129920..130483 - 564 WP_249530946 TrbI/VirB10 family protein traB
RG72_RS26260 (RG72_24860) 130529..132142 - 1614 WP_016236744 IS66-like element ISEc23 family transposase -
RG72_RS26265 (RG72_24865) 132173..132523 - 351 WP_000624722 IS66 family insertion sequence element accessory protein TnpB -
RG72_RS26270 (RG72_24870) 132520..132945 - 426 WP_000422741 transposase -
RG72_RS26275 (RG72_24875) 133030..133911 - 882 Protein_160 F-type conjugal transfer pilus assembly protein TraB -
RG72_RS26280 (RG72_24880) 133911..134639 - 729 WP_001230772 type-F conjugative transfer system secretin TraK traK
RG72_RS26285 (RG72_24885) 134626..135192 - 567 WP_000399780 type IV conjugative transfer system protein TraE traE
RG72_RS26290 (RG72_24890) 135214..135525 - 312 WP_000012113 type IV conjugative transfer system protein TraL traL
RG72_RS26295 (RG72_24895) 135540..135899 - 360 WP_001098992 type IV conjugative transfer system pilin TraA -
RG72_RS26300 (RG72_24900) 135932..136159 - 228 WP_000089263 conjugal transfer relaxosome protein TraY -
RG72_RS26305 (RG72_24905) 136296..136967 - 672 WP_000283561 conjugal transfer transcriptional regulator TraJ -
RG72_RS26310 (RG72_24910) 137161..137544 - 384 WP_001354030 conjugal transfer relaxosome DNA-binding protein TraM -
RG72_RS26315 (RG72_24915) 137867..138469 + 603 WP_000243713 transglycosylase SLT domain-containing protein virB1
RG72_RS26320 (RG72_24920) 138766..139587 - 822 WP_001234445 DUF932 domain-containing protein -
RG72_RS26325 (RG72_24925) 139698..139994 - 297 WP_001272251 hypothetical protein -
RG72_RS28190 (RG72_24930) 140294..140590 + 297 Protein_171 hypothetical protein -
RG72_RS26345 (RG72_24935) 140909..141034 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
RG72_RS28630 (RG72_24935) 140976..141125 - 150 Protein_173 plasmid maintenance protein Mok -
RG72_RS28195 (RG72_24940) 141147..141326 + 180 WP_001309233 hypothetical protein -
RG72_RS26355 (RG72_24945) 141304..142066 - 763 Protein_175 plasmid SOS inhibition protein A -
RG72_RS26360 (RG72_24950) 142063..142497 - 435 WP_000845953 conjugation system SOS inhibitor PsiB -

Host bacterium

ID   1147 GenBank   NZ_CP010239
Plasmid name   pS50-A Incompatibility group   Col156
Plasmid size   184614 bp Coordinate of oriT [Strand]   137532..138081 [+]
Host baterium   Escherichia coli strain S50

Cargo genes

Drug resistance gene   ethidium bromide resistance; quinolone resistance
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -