Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100676
• Name: oriT_pH8-C
• Organism: Escherichia coli strain H8
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NZ_CP010175 (5888..5970 [+], 83 nt)
• oriT length: 83 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 83 nt

>oriT_pH8-C
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Nuclease_pH8-C

• ID: 748
• GenBank: WP_000635804
• Name: Nuclease_pH8-C
• UniProt ID: _
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57794.27 Da
• Isoelectric Point: 6.0811

>WP_000635804.1 MULTISPECIES: nuclease [Enterobacteriaceae]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRR
YSPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRN
QEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIQE
REHVLSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

No available structure.

Host bacterium

• ID: 1136
• GenBank: NZ_CP010175
• Plasmid name: pH8-C
• Incompatibility group: ColRNAI
• Plasmid size: 6647 bp
• Coordinate of oriT [Strand]: 5888..5970 [+]
• Host baterium: Escherichia coli strain H8

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -