Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   100676
Name   oriT_pH8-C in_silico
Organism   Escherichia coli strain H8
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP010175 (5888..5970 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)      3..12, 16..25  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site      56..57
Conserved sequence flanking the
  nic site  
 
 CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 83 nt

>oriT_pH8-C
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   748 GenBank   WP_000635804
Name   Nuclease_pH8-C insolico UniProt ID   _
Length   517 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 517 a.a.        Molecular weight: 57794.27 Da        Isoelectric Point: 6.0811

>WP_000635804.1 MULTISPECIES: nuclease [Enterobacteriaceae]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRR
YSPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRN
QEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIQE
REHVLSLERERQPEIQERTLDGPSLGW

  Protein domains


Predicted by InterproScan.

(57-237)


  Protein structure



No available structure.




Host bacterium


ID   1136 GenBank   NZ_CP010175
Plasmid name   pH8-C Incompatibility group   ColRNAI
Plasmid size   6647 bp Coordinate of oriT [Strand]   5888..5970 [+]
Host baterium   Escherichia coli strain H8

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -