Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100674
Name   oriT_pH3-A in_silico
Organism   Escherichia coli strain H3
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP010168 (17708..18061 [+], 354 nt)
oriT length   354 nt
IRs (inverted repeats)      IR1: 145..155, 161..171  (ATGTGTAATAC..GTATTACACAT)
  IR2: 208..218, 222..232  (GCATTTACGAT..ATCGTAATTGC)
  IR3: 335..343, 346..354  (CTTTCATGC..GCATGAAAG)
Location of nic site      241..242
Conserved sequence flanking the
  nic site    		  GGTGT|ATGGC
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 354 nt

>oriT_pH3-A
ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCTCTTCGGTCACTCTCAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACAGATGTAATACATACAAATCTAGCATACATGCGGCTTAGAACCACATGTGTAATACGTTGCGTATTACACATTAAAACACAAATTATAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAATCGTAATTGCGTATGGTGTATGGCCATTAAGGGATACCATACCACGCCTTTTTTAAGGGAGAAACCGGTGTTACGTGCAAGTAAATCGCTCAAAAAGCGTTCACATTCACCACTTTCATGCTTGCATGAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   746 GenBank   WP_052989320
Name   Relaxase_pH3-A insolico UniProt ID   _
Length   968 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 968 a.a.        Molecular weight: 108374.46 Da        Isoelectric Point: 9.7662

>WP_052989320.1 MULTISPECIES: MobF family relaxase [Enterobacteriaceae]
MLNITTITRQRVDRVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLDGKVTDDISVM
RKTANSESKERLGYDLTFSAPKGVSLQALVNGDKRIIEAHDRAVTKAIEEAERLALGRFTVNKKTHVENT
NKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSLTNDGIVNSLTLLGNVYKSELARELELAGYN
LRYDRNGTFDLAHFSQEQIAQFSQRSQQIEAELAKNGLSRETASHELKNAAALKTRKSKGEVDRDVLYEG
WKNRAKELQIDFDSREWKGAANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAM
KHGYGRLTLDDIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRSKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQLKAVMGIIHT
PNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEGLESRTLQSFLRASDKKIGPG
SVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTKAVEAGKPFEQLIKAGMETAYMKDIQRQKDP
ELLKAALNAAEGKIKASLTHVTSIVEEKNHDQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINEYIQ
AELGLKGQGINYPLLNRLDTTQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGS
GDTIAFSPARFSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKSVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDEKKLDKAASRE
GFKTAALELEQLKRYAKEQQHDTREKGRDKAEPTQDKGNARPKDKGHEKNNDDRALRL

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(14-287)

AAA domain (AAA_30)

(475-649)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(850-896)


  Protein structure



No available structure.




Host bacterium

ID   1134 GenBank   NZ_CP010168
Plasmid name   pH3-A Incompatibility group   IncX1
Plasmid size   48243 bp Coordinate of oriT [Strand]   17708..18061 [+]
Host baterium   Escherichia coli strain H3

Cargo genes

Drug resistance gene   tetracycline resistance; quinolone resistance
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -