Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100673
Name   oriT_pD10-B in_silico
Organism   Escherichia coli strain D10
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP010159 (3102..3178 [-], 77 nt)
oriT length   77 nt
IRs (inverted repeats)      3..12, 16..25  (GTGTCGGGGC..GCCATGACCC)
Location of nic site      56..57
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 77 nt

>oriT_pD10-B
GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAATCATGTGGCATCAGTG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   745 GenBank   WP_044783502
Name   PutativerelaxaseofpD10-B insolico UniProt ID   _
Length   532 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 532 a.a.        Molecular weight: 59119.62 Da        Isoelectric Point: 8.3326

>WP_044783502.1 relaxase/mobilization nuclease domain-containing protein [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERSREGARVLRGAPEEVRELIDATPFAKKYTSGVLSFAEQTLPPGER
ERVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELASGKRLQPYYDRADRPRINAWQTLVN
HHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEALYHAGALKTRQDVTEALTAAGFEVVRTTRS
SISIADPEGGRNLRLKGALYEQSFTNGDRLREETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRR
YQRTHSSGERTTGDAEQCRERAVQRHRERTGPAAGRAALEAGQRADTVPVADGALRHGRDFSGVMGADSV
AGKPDCGEHPGYQGPGRDAGETPGKNMGCDVSRGQEREISGVAVRGEGGKQLDGGEEERSKTGQGVSEYD
RAGNALTERLRAIAAGLYAAAERLGERLHGFAAYVRADTTGERATEGTVRRLESAGAEISRAGGPLESVI
YHEQQIRDEQERQARLERERARSWHEEQEREARVWRGPSMGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(58-239)


  Protein structure



No available structure.




Host bacterium

ID   1133 GenBank   NZ_CP010159
Plasmid name   pD10-B Incompatibility group   ColRNAI
Plasmid size   6822 bp Coordinate of oriT [Strand]   3102..3178 [-]
Host baterium   Escherichia coli strain D10

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -