Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100665
Name   oriT_pBCO_5 in_silico
Organism   Bacillus cereus E33L
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NZ_CP009965 (3525..3563 [+], 39 nt)
oriT length   39 nt
IRs (inverted repeats)      1..8, 9..16  (CTTTACGA..TAGTAAAG)
Location of nic site      25..26
Conserved sequence flanking the
  nic site    		  GTG|CTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 39 nt

>oriT_pBCO_5
CTTTACGATAGTAAAGTATAGTGTGCTATACTTTACATG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   737 GenBank   WP_000119308
Name   PutativerelaxaseofpBCO_5 insolico UniProt ID   Q4V103
Length   411 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 411 a.a.        Molecular weight: 48609.55 Da        Isoelectric Point: 9.5479

>WP_000119308.1 MobV family relaxase [Bacillus cereus]
MSYAICRMQKFKMRDVKGIQIHNQREKESHTNHDIESERTHLNYDLHNDGHIDYLKTVKDKIEQNVETNR
AIRKDAVVMCEFVVTSDKEFFDVLDMVDPVQQKVFFKEAYNFLKNRYGEQNIVHAAVHLDEKTPHMHVGM
VPVTEENKLSAKQIFNRKELVSLQDDFHAHMVEKGFYLERGVSSDKKHVEMRKLKAMTAKEEIKALEKEM
GQCLQKKEVVNKQMEQLESRLNQLQNAVKSTNKVDKMEVKHKGGILRSKTVEMSVEDFEELKTKAKASEA
FKRENMTLHRKNEALQASNTDLHTTVKRLEKENEGLKPYKARYERLAKLMDEMNKFYEKFIPKELSKFHE
IIGFCKRKVNASINRFSSLRYSEKELNENEKRGYETASKFLENQRKEQKQQRKERGDEREL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-195)


  Protein structure


Source ID Structure
AlphaFold DB Q4V103


Host bacterium

ID   1125 GenBank   NZ_CP009965
Plasmid name   pBCO_5 Incompatibility group   _
Plasmid size   4983 bp Coordinate of oriT [Strand]   3525..3563 [+]
Host baterium   Bacillus cereus E33L

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -