Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100664
Name   oriT_pKLDS1.8701 in_silico
Organism   Lactobacillus helveticus strain KLDS1.8701
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP009908 (7173..7635 [-], 463 nt)
oriT length   463 nt
IRs (inverted repeats)      IR1: 138..147, 152..161  (CCCATTTTAT..ATAAAATGGG)
  IR2: 207..219, 223..235  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 242..255, 268..281  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site      290..291
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 463 nt

>oriT_pKLDS1.8701
TTAGTGGATGAACAAATAAAATACGAGAGATTTTTTGTTCATTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGGAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATCTATGGCTTTATATGGTCTATTTTGTTATAATAATTGTAACCGAATAGGGCGCAATGATTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATTTATGGACCAGAAAGAAGTATCACAAAATCAAACAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   736 GenBank   WP_052541636
Name   Relaxase_pKLDS1.8701 insolico UniProt ID   A0A0D5ML92
Length   410 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47787.63 Da        Isoelectric Point: 9.2964

>WP_052541636.1 relaxase/mobilization nuclease domain-containing protein [Lactobacillus helveticus]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLDTGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQYVSVSSYKTFKECLSDNGVILSERGKTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQREPPITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQFAGRLPELTQ
RVARKLKQTKEKILDDFERRFSKDMKNYEQEQQKILEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   1124 GenBank   NZ_CP009908
Plasmid name   pKLDS1.8701 Incompatibility group   _
Plasmid size   10600 bp Coordinate of oriT [Strand]   7173..7635 [-]
Host baterium   Lactobacillus helveticus strain KLDS1.8701

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -