Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 100659 |
Name | oriT_pKPC-629 |
Organism | Escherichia coli strain ECONIH1 |
Sequence Completeness | intact |
NCBI accession of oriT (coordinates [strand]) | NZ_CP009862 (66234..66860 [+], 627 nt) |
oriT length | 627 nt |
IRs (inverted repeats) | IR1: 198..207, 211..220 (ATTGGGTGTT..AATACCTAAT) IR2:319..324, 330..335 (AAAAAA..TTTTTT) IR3: 393..402, 405..414 (GTATTCATGC..GCATGAATAC) |
Location of nic site | 301..302 |
Conserved sequence flanking the nic site |
GGTGT|ATAGC |
Note | predicted by the oriTfinder |
oriT sequence
Download Length: 627 nt
>oriT_pKPC-629
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 731 | GenBank | WP_001144737 |
Name | MobC_pKPC-629 | UniProt ID | T2FLB2 |
Length | 88 a.a. | PDB ID | |
Note | putative relaxase |
Relaxase protein sequence
Download Length: 88 a.a. Molecular weight: 10315.57 Da Isoelectric Point: 5.5791
>WP_001144737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Gammaproteobacteria]
MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLKQEAIEKLAERIDQHLDHVSELFDKTW
SRWHDCHFQKTTAPVDWA
MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLKQEAIEKLAERIDQHLDHVSELFDKTW
SRWHDCHFQKTTAPVDWA
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | T2FLB2 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 11795..22120
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
ECONIH1_RS27530 (ECONIH1_27530) | 7640..9073 | - | 1434 | WP_001288432 | DNA cytosine methyltransferase | - |
ECONIH1_RS27535 (ECONIH1_27535) | 9172..9444 | + | 273 | Protein_10 | IS1 family transposase | - |
ECONIH1_RS27540 (ECONIH1_27540) | 9455..9661 | - | 207 | WP_001749967 | hypothetical protein | - |
ECONIH1_RS27545 (ECONIH1_27545) | 9666..10313 | - | 648 | WP_012561184 | restriction endonuclease | - |
ECONIH1_RS27550 (ECONIH1_27550) | 10363..10674 | - | 312 | WP_001452736 | hypothetical protein | - |
ECONIH1_RS27555 (ECONIH1_27555) | 10710..11024 | - | 315 | WP_001749965 | TrbM/KikA/MpfK family conjugal transfer protein | - |
ECONIH1_RS27560 (ECONIH1_27560) | 11021..11365 | - | 345 | WP_001749964 | hypothetical protein | - |
ECONIH1_RS27565 (ECONIH1_27565) | 11381..11686 | - | 306 | WP_000960954 | H-NS family nucleoid-associated regulatory protein | - |
ECONIH1_RS27570 (ECONIH1_27570) | 11795..12529 | + | 735 | WP_001749963 | lytic transglycosylase domain-containing protein | virB1 |
ECONIH1_RS27575 (ECONIH1_27575) | 12538..12819 | + | 282 | WP_000440698 | transcriptional repressor KorA | - |
ECONIH1_RS27580 (ECONIH1_27580) | 12829..13122 | + | 294 | WP_001749962 | hypothetical protein | virB2 |
ECONIH1_RS27585 (ECONIH1_27585) | 13172..13489 | + | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
ECONIH1_RS27590 (ECONIH1_27590) | 13489..16089 | + | 2601 | WP_040123405 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
ECONIH1_RS27595 (ECONIH1_27595) | 16107..16820 | + | 714 | WP_001749960 | type IV secretion system protein | virB5 |
ECONIH1_RS27600 (ECONIH1_27600) | 16828..17055 | + | 228 | WP_001749959 | IncN-type entry exclusion lipoprotein EexN | - |
ECONIH1_RS27605 (ECONIH1_27605) | 17071..18111 | + | 1041 | WP_001749958 | type IV secretion system protein | virB6 |
ECONIH1_RS27610 (ECONIH1_27610) | 18194..18340 | + | 147 | WP_001257173 | conjugal transfer protein TraN | - |
ECONIH1_RS27615 (ECONIH1_27615) | 18330..19028 | + | 699 | WP_000646594 | type IV secretion system protein | virB8 |
ECONIH1_RS27620 (ECONIH1_27620) | 19039..19923 | + | 885 | WP_000735066 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
ECONIH1_RS27625 (ECONIH1_27625) | 19923..21083 | + | 1161 | WP_000101710 | type IV secretion system protein VirB10 | virB10 |
ECONIH1_RS27630 (ECONIH1_27630) | 21125..22120 | + | 996 | WP_000128596 | ATPase, T2SS/T4P/T4SS family | virB11 |
ECONIH1_RS27635 (ECONIH1_27635) | 22120..22653 | + | 534 | WP_000792636 | phospholipase D family protein | - |
ECONIH1_RS32755 (ECONIH1_27640) | 22827..23114 | - | 288 | Protein_31 | DUF6710 family protein | - |
ECONIH1_RS30190 | 23381..23560 | + | 180 | Protein_32 | helix-turn-helix domain-containing protein | - |
ECONIH1_RS30195 | 23625..24185 | + | 561 | Protein_33 | IS6-like element IS26 family transposase | - |
ECONIH1_RS27650 (ECONIH1_27650) | 24205..26316 | - | 2112 | Protein_34 | Tn3-like element Tn3 family transposase | - |
Host bacterium
ID | 1119 | GenBank | NZ_CP009862 |
Plasmid name | pKPC-629 | Incompatibility group | IncN |
Plasmid size | 80186 bp | Coordinate of oriT [Strand] | 66234..66860 [+] |
Host baterium | Escherichia coli strain ECONIH1 |
Cargo genes
Drug resistance gene | dfrA14, blaKPC-2, aac(6')-Ib, ant(3'')-Ia, blaOXA-9, blaTEM-1A, aph(6)-Id, aph(3'')-Ib, sul2 |
Virulence gene | - |
Metal resistance gene | merR, merT, merP, merA, merD, merE |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |