Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100650
Name   oriT_pCVMN1543 in_silico
Organism   Salmonella enterica subsp. enterica serovar Newport str. CVM N1543 isolate CFSAN000926
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP009570 (21401..21951 [-], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pCVMN1543
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   722 GenBank   WP_001326173
Name   TraI_pCVMN1543 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12497..23817

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SEEN543_RS00065 (SEEN543_26040) 7860..9047 + 1188 WP_000219627 hypothetical protein -
SEEN543_RS00070 (SEEN543_26045) 9047..10531 + 1485 WP_000124148 hypothetical protein -
SEEN543_RS00075 (SEEN543_26050) 10558..11409 - 852 WP_000248802 phage repressor protein C1 -
SEEN543_RS00080 (SEEN543_26055) 11518..11727 - 210 WP_000864014 hypothetical protein -
SEEN543_RS00090 (SEEN543_26065) 12623..12829 + 207 WP_000968892 hypothetical protein -
SEEN543_RS00095 (SEEN543_26070) 12853..13923 + 1071 WP_001533532 hypothetical protein -
SEEN543_RS00100 (SEEN543_26075) 13920..14135 + 216 WP_000804048 hypothetical protein -
SEEN543_RS00105 (SEEN543_26080) 14143..15171 + 1029 WP_000185184 tyrosine-type recombinase/integrase -
SEEN543_RS00110 (SEEN543_26085) 15206..15568 + 363 WP_001533519 hypothetical protein -
SEEN543_RS00115 (SEEN543_26090) 15565..15723 + 159 WP_001252573 hypothetical protein -
SEEN543_RS00120 (SEEN543_26095) 15838..16458 + 621 WP_000527000 hypothetical protein -
SEEN543_RS00125 (SEEN543_26100) 16445..16888 + 444 WP_000169636 phage protein NinX family protein -
SEEN543_RS00130 (SEEN543_26105) 16891..17202 + 312 WP_000934524 hypothetical protein -
SEEN543_RS00135 (SEEN543_26110) 17335..17886 + 552 WP_024135640 Ref family recombination enhancement nuclease -
SEEN543_RS00140 (SEEN543_26115) 17914..18147 + 234 WP_000354002 hypothetical protein -
SEEN543_RS00145 (SEEN543_26120) 18353..19003 + 651 WP_000933936 DUF2829 domain-containing protein -
SEEN543_RS00150 (SEEN543_26125) 19101..19679 + 579 WP_023226264 hypothetical protein -
SEEN543_RS00155 (SEEN543_26130) 19736..20071 - 336 WP_000806443 HigA family addiction module antitoxin -
SEEN543_RS00160 (SEEN543_26135) 20133..20435 - 303 WP_000037759 type II toxin-antitoxin system RelE/ParE family toxin -
SEEN543_RS00165 (SEEN543_26140) 20710..21351 + 642 WP_024133281 hypothetical protein -
SEEN543_RS00170 (SEEN543_26145) 21520..21897 + 378 WP_000235963 hypothetical protein -
SEEN543_RS00175 (SEEN543_26150) 21910..23127 + 1218 WP_001143860 hypothetical protein -
SEEN543_RS00180 (SEEN543_26155) 23131..23871 + 741 WP_000896790 hypothetical protein -
SEEN543_RS00185 (SEEN543_26160) 23855..24646 + 792 WP_000224251 hypothetical protein -
SEEN543_RS00190 (SEEN543_26165) 24675..25691 + 1017 WP_000212013 hypothetical protein -
SEEN543_RS00195 (SEEN543_26170) 25684..26292 + 609 WP_000527468 hypothetical protein -
SEEN543_RS00200 (SEEN543_26175) 26423..26626 + 204 WP_000167440 hypothetical protein -

Host bacterium

ID   1110 GenBank   NZ_CP009570
Plasmid name   pCVMN1543 Incompatibility group   IncA/C2
Plasmid size   85837 bp Coordinate of oriT [Strand]   21401..21951 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Newport str. CVM N1543 isolate CFSAN000926

Cargo genes

Drug resistance gene   resistance to cetylpyridinium; quaternary ammonium compound-resistance
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merB, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -