Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   100642
Name   oriT_pKEC-dc3 in_silico
Organism   Klebsiella pneumoniae subsp. pneumoniae KPNIH27
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP007732 (92240..92790 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pKEC-dc3
GAGCAGAGCTATGTGTGACAAGAAGTATAGAGATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   714 GenBank   WP_012561166
Name   Relaxase_pKEC-dc3 insolico UniProt ID   A0A023UF58
Length   1078 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

(851-895)

(16-287)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB A0A023UF58


T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 90374..116904

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH27_RS26755 (KPNIH27_26045) 86399..86632 - 234 WP_001191892 hypothetical protein -
KPNIH27_RS26760 (KPNIH27_26050) 86614..87231 - 618 WP_001249396 hypothetical protein -
KPNIH27_RS26765 (KPNIH27_26055) 87399..90377 + 2979 WP_001337757 MobH family relaxase -
KPNIH27_RS26770 (KPNIH27_26060) 90374..92239 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
KPNIH27_RS26775 (KPNIH27_26065) 92289..92834 + 546 WP_000228720 hypothetical protein -
KPNIH27_RS26780 (KPNIH27_26070) 92791..93420 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
KPNIH27_RS26785 (KPNIH27_26075) 93430..93876 + 447 WP_000122506 hypothetical protein -
KPNIH27_RS26790 (KPNIH27_26080) 93886..94065 + 180 WP_041445762 hypothetical protein -
KPNIH27_RS26800 (KPNIH27_26090) 94154..95358 + 1205 WP_086008128 IS3 family transposase -
KPNIH27_RS26805 (KPNIH27_26095) 95422..95601 + 180 WP_228703103 hypothetical protein -
KPNIH27_RS26810 (KPNIH27_26100) 95601..96263 + 663 WP_001231463 hypothetical protein -
KPNIH27_RS34875 96444..96587 + 144 WP_001275801 hypothetical protein -
KPNIH27_RS26815 (KPNIH27_26105) 96599..96964 + 366 WP_001052531 hypothetical protein -
KPNIH27_RS26820 (KPNIH27_26110) 97109..97390 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
KPNIH27_RS26825 (KPNIH27_26115) 97387..98013 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
KPNIH27_RS26830 (KPNIH27_26120) 97997..98914 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
KPNIH27_RS26835 (KPNIH27_26125) 98914..100227 + 1314 WP_024131605 TraB/VirB10 family protein traB
KPNIH27_RS26840 (KPNIH27_26130) 100224..100802 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
KPNIH27_RS26845 (KPNIH27_26135) 100806..101198 + 393 WP_000479535 TraA family conjugative transfer protein -
KPNIH27_RS26850 (KPNIH27_26140) 101399..106930 + 5532 WP_000606833 Ig-like domain-containing protein -
KPNIH27_RS26855 (KPNIH27_26145) 107080..107787 + 708 WP_001259347 DsbC family protein -
KPNIH27_RS26860 (KPNIH27_26150) 107784..110231 + 2448 WP_000637386 type IV secretion system protein TraC virb4
KPNIH27_RS26865 (KPNIH27_26155) 110246..110563 + 318 WP_000351984 hypothetical protein -
KPNIH27_RS26870 (KPNIH27_26160) 110560..111090 + 531 WP_001010738 S26 family signal peptidase -
KPNIH27_RS26875 (KPNIH27_26165) 111053..112318 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
KPNIH27_RS26880 (KPNIH27_26170) 112315..112986 + 672 WP_001337754 EAL domain-containing protein -
KPNIH27_RS26885 (KPNIH27_26175) 112986..113990 + 1005 WP_043940352 TraU family protein traU
KPNIH27_RS26890 (KPNIH27_26180) 114106..116904 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
KPNIH27_RS26895 (KPNIH27_26185) 116943..117803 - 861 WP_041445776 hypothetical protein -
KPNIH27_RS26900 (KPNIH27_26190) 117926..118567 - 642 WP_000796664 hypothetical protein -
KPNIH27_RS26905 (KPNIH27_26195) 118861..119181 + 321 WP_000547566 hypothetical protein -
KPNIH27_RS26910 (KPNIH27_26200) 119485..119670 + 186 WP_001186917 hypothetical protein -
KPNIH27_RS26915 (KPNIH27_26205) 119890..120858 + 969 WP_000085162 AAA family ATPase -
KPNIH27_RS26920 (KPNIH27_26210) 120869..121777 + 909 WP_000739139 hypothetical protein -


Host bacterium


ID   1102 GenBank   NZ_CP007732
Plasmid name   pKEC-dc3 Incompatibility group   IncA/C2
Plasmid size   268334 bp Coordinate of oriT [Strand]   92240..92790 [+]
Host baterium   Klebsiella pneumoniae subsp. pneumoniae KPNIH27

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   mercury resistance
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -