Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100635
Name   oriT_pOV14c in_silico
Organism   Ensifer adhaerens OV14
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NZ_CP007238 (52599..52642 [+], 44 nt)
oriT length   44 nt
IRs (inverted repeats)      21..27, 32..38  (ACGTCGC..GCGACGT)
Location of nic site      11..12
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 44 nt

>oriT_pOV14c
GGATCCAAGGGCGCAATTATACGTCGCTGATGCGACGTGCTTGC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   707 GenBank   WP_025430013
Name   TraA_pOV14c insolico UniProt ID   W8ID92
Length   1100 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 123851.52 Da        Isoelectric Point: 9.6978

>WP_025430013.1 Ti-type conjugative transfer relaxase TraA [Ensifer adhaerens]
MAIAHFSASIVSRGGGRSVVLSAAYRHCAKMEYEREARTIDYTRKEGLLHEEFMLPADAPKWVRAMIADR
SVAGASEAFWNKVEAFEKRSDAQLARDLTIALPLELTPEQNIALVRDFVERHIHAKGMVADWVYHDNPGN
PHIHLMTTLRPLTEEGFGSKKVAVNGEDGQPVRTKSGKILYELWAGSTDDFNSLRDGWFERLNHHLALGG
IDLRIDGRSYEKQGIELEPTIHLGVGAKAIERKAQEQGVRPELERIELNKERRSENARRILRNPGIVVDL
ITREKSVFDERDVAKVLHRYVDDPTVFQQLMLRIILNPEVLRLQRDTIEFATGEKVPARYSTRAMIRLEA
TMVRQALWLSNRDGHAVSDAALDVTFRRHERLSQEQKAAIERIAGPARIAAVVGRAGAGKTTMMKAAREV
WELAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWNRGRDVLDDKTVFVMDEAGMVASKQMADFVD
AVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVNRIGYAELETIYRQREDWMRKASLDLARGNVEKALTAYD
ANIRITGTRLKAEAVERLIGDWNHDYDQTKTTLILAHLRRDVRMLNVMAREKLVERGIVGEGHVFKTADG
IRQFDAGDQIVFLKNEGLLGVKNGMIAHVTEAAPNRIVAVIGEGDQRRQVIVEQRFYSNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGHRSFAFNGGLAKVLSRKNAKETTLDYERGKLY
REALRYAENRGLNIVQVARTLLRDQLDWTLRQKTKLVDLGQRLAVFAERLGLHHPPKTQTMKEAAPMVAG
IKTFSGSVADTVEHKLDTDPALRQQWEEVSTRFVYVFADPETAFRAMNFDAVLVDKETAKLALQTLTTAP
ASIGPLRGKTGILASKTEREDRRVADLNVPALKRDLEQYLRMRETAVQRLQADEQVLRQRVSIDIPALSP
AAHVVLERVRDAIDRNDLPAAMAYALSNRETKLEIDGFNKAVTERFGERTLLTNAAREPSGKLFDKLSEG
MQPEQKERLTEAWPIMRASQQLAAHERTVQTLRQAEDLRLAPRQTPVMKQ

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-243)

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(692-736)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-951)

AAA domain (AAA_30)

(382-567)


  Protein structure


Source ID Structure
AlphaFold DB W8ID92


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 102429..112118

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OV14_RS40265 98056..98535 + 480 WP_081789021 ATP-binding cassette domain-containing protein -
OV14_RS28745 (OV14_c0086) 98642..99397 + 756 WP_025430053 amino acid ABC transporter ATP-binding protein -
OV14_RS28750 (OV14_c0087) 99457..100764 + 1308 WP_025430054 aminotransferase class III-fold pyridoxal phosphate-dependent enzyme -
OV14_RS28755 100781..101155 + 375 WP_025430055 nuclear transport factor 2 family protein -
OV14_RS37495 (OV14_c0089) 101283..102257 + 975 WP_081789023 DMT family transporter -
OV14_RS28765 (OV14_c0090) 102429..103748 - 1320 WP_025430057 IncP-type conjugal transfer protein TrbI virB10
OV14_RS28770 (OV14_c0091) 103760..104236 - 477 WP_025430058 conjugal transfer protein TrbH -
OV14_RS28775 (OV14_c0092) 104236..105087 - 852 WP_025430059 P-type conjugative transfer protein TrbG virB9
OV14_RS28780 (OV14_c0093) 105105..105782 - 678 WP_025430060 conjugal transfer protein TrbF virB8
OV14_RS28785 (OV14_c0094) 105800..106990 - 1191 WP_025430061 P-type conjugative transfer protein TrbL virB6
OV14_RS28790 106984..107211 - 228 WP_025430062 entry exclusion protein TrbK -
OV14_RS28795 (OV14_c0096) 107208..107957 - 750 WP_244445490 P-type conjugative transfer protein TrbJ virB5
OV14_RS28800 (OV14_c0097) 107989..110457 - 2469 WP_025430064 conjugal transfer protein TrbE virb4
OV14_RS28805 (OV14_c0098) 110461..110760 - 300 WP_025430065 conjugal transfer protein TrbD virB3
OV14_RS28810 (OV14_c0099) 110753..111157 - 405 WP_025430066 conjugal transfer pilin TrbC virB2
OV14_RS28815 (OV14_c0100) 111147..112118 - 972 WP_025430067 P-type conjugative transfer ATPase TrbB virB11
OV14_RS28820 (OV14_c0101) 112115..112750 - 636 WP_025430068 acyl-homoserine-lactone synthase -
OV14_RS28825 (OV14_c0103) 113128..114318 + 1191 WP_025430069 plasmid partitioning protein RepA -
OV14_RS28830 (OV14_c0104) 114403..115398 + 996 WP_025430070 plasmid partitioning protein RepB -
OV14_RS28835 (OV14_c0105) 115554..116798 + 1245 WP_025430071 plasmid replication protein RepC -

Host bacterium

ID   1095 GenBank   NZ_CP007238
Plasmid name   pOV14c Incompatibility group   _
Plasmid size   125203 bp Coordinate of oriT [Strand]   52599..52642 [+]
Host baterium   Ensifer adhaerens OV14

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -