Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100631
Name   oriT_pO145-12761 in_silico
Organism   Escherichia coli O145:H28 str. RM12761
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP007135 (58313..58762 [+], 450 nt)
oriT length   450 nt
IRs (inverted repeats)      130..139, 142..151  (ATAGGGTCGT..ACGCCCCTAT)
 219..226, 229..236  (GCAAAAAC..GTTTTTGC)
Location of nic site      245..246
Conserved sequence flanking the
  nic site    		  GTGGGGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 450 nt

>oriT_pO145-12761
CCTAGCAGCGCCCCTAGGGTCATACTATAAAAAAACGCACGGCGCTGCCAGGGGCGGCCCTAACATATCCAAATGTTTTTCATGAAAATTGTCAGTACTGACCCTAACAAGGGTCGTCATAGGGTCGCCATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAAATTAAAATACATTATTCATAACATAAGCTAATGATTCAAAAAACAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCAGGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   703 GenBank   WP_025380714
Name   TraI_pO145-12761 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192053.57 Da        Isoelectric Point: 5.9267

>WP_025380714.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPASIISEPDRNVRYTRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHQEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFTELGREIDA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLSASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIVGISKDDGLIILQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVSLQGSRNGESLLADNMQEGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(10-283)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-627)

DNA helicase TraI (TraI_C)

(1434-1557)

AAA domain (AAA_30)

(968-1156)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 43388..59158

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12761_RS28050 (ECRM12761_27710) 43388..45541 - 2154 WP_001369365 type IV conjugative transfer system coupling protein TraD virb4
ECRM12761_RS28055 (ECRM12761_27715) 45793..46524 - 732 WP_000782454 conjugal transfer complement resistance protein TraT -
ECRM12761_RS28060 46573..47058 - 486 WP_000605868 hypothetical protein -
ECRM12761_RS28065 (ECRM12761_27725) 47074..49635 - 2562 Protein_50 conjugal transfer mating-pair stabilization protein TraG -
ECRM12761_RS28075 49685..50898 + 1214 WP_136952279 IS3 family transposase -
ECRM12761_RS33055 50945..51214 - 270 Protein_52 hypothetical protein -
ECRM12761_RS30970 (ECRM12761_27735) 51207..51428 - 222 WP_001278689 conjugal transfer protein TraR -
ECRM12761_RS28085 (ECRM12761_27740) 51563..52078 - 516 WP_021532964 type IV conjugative transfer system lipoprotein TraV traV
ECRM12761_RS28090 (ECRM12761_27745) 52075..52326 - 252 WP_001038348 conjugal transfer protein TrbG -
ECRM12761_RS28095 (ECRM12761_27750) 52319..52639 - 321 WP_025380715 conjugal transfer protein TrbD virb4
ECRM12761_RS28100 (ECRM12761_27755) 52626..53213 - 588 WP_025380716 conjugal transfer pilus-stabilizing protein TraP -
ECRM12761_RS28105 (ECRM12761_27760) 53203..54630 - 1428 WP_025380717 F-type conjugal transfer pilus assembly protein TraB traB
ECRM12761_RS28110 (ECRM12761_27765) 54630..55358 - 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
ECRM12761_RS28115 (ECRM12761_27770) 55345..55911 - 567 WP_000399792 type IV conjugative transfer system protein TraE traE
ECRM12761_RS28120 (ECRM12761_27775) 55933..56244 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
ECRM12761_RS28125 (ECRM12761_27780) 56259..56618 - 360 WP_000340272 type IV conjugative transfer system pilin TraA -
ECRM12761_RS28130 (ECRM12761_27785) 56652..56879 - 228 WP_001254386 conjugal transfer relaxosome protein TraY -
ECRM12761_RS28135 (ECRM12761_27790) 56974..57660 - 687 WP_025380718 PAS domain-containing protein -
ECRM12761_RS28140 (ECRM12761_27795) 57851..58234 - 384 WP_000124981 conjugal transfer relaxosome DNA-binding protein TraM -
ECRM12761_RS28145 (ECRM12761_27800) 58511..59158 + 648 WP_122994606 transglycosylase SLT domain-containing protein virB1
ECRM12761_RS28150 (ECRM12761_27810) 59455..60276 - 822 WP_001234426 DUF932 domain-containing protein -
ECRM12761_RS28155 (ECRM12761_27815) 60394..60681 - 288 WP_000107535 hypothetical protein -
ECRM12761_RS33570 60706..60912 - 207 WP_000547968 hypothetical protein -
ECRM12761_RS33575 60982..61155 + 174 Protein_70 hypothetical protein -
ECRM12761_RS33580 (ECRM12761_27820) 61153..61383 - 231 WP_001426396 hypothetical protein -
ECRM12761_RS28170 61603..61728 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
ECRM12761_RS33585 61670..61819 - 150 Protein_73 plasmid maintenance protein Mok -
ECRM12761_RS32820 (ECRM12761_27825) 61841..62029 + 189 WP_001299721 hypothetical protein -
ECRM12761_RS28180 (ECRM12761_27830) 61998..62760 - 763 Protein_75 plasmid SOS inhibition protein A -
ECRM12761_RS28185 (ECRM12761_27835) 62757..63191 - 435 WP_025380720 conjugation system SOS inhibitor PsiB -
ECRM12761_RS30980 (ECRM12761_27840) 63246..63443 - 198 Protein_77 hypothetical protein -
ECRM12761_RS28190 (ECRM12761_27845) 63471..63704 - 234 WP_025380721 DUF905 family protein -

Host bacterium

ID   1091 GenBank   NZ_CP007135
Plasmid name   pO145-12761 Incompatibility group   IncFIB
Plasmid size   98067 bp Coordinate of oriT [Strand]   58313..58762 [+]
Host baterium   Escherichia coli O145:H28 str. RM12761

Cargo genes

Drug resistance gene   -
Virulence gene   stcE, exeE, exeG, hlyC, hlyA, hlyB, hlyD
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -