Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100624
Name   oriT_PCN061p2 in_silico
Organism   Escherichia coli PCN061
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP006638 (1625..1810 [-], 186 nt)
oriT length   186 nt
IRs (inverted repeats)      14..21, 25..32  (TTCGGGGC..GCCCTGAA)
Location of nic site      65..66
Conserved sequence flanking the
  nic site    		  CTGG|CTCA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 186 nt

>oriT_PCN061p2
GGTTTTAGCAGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTCAATATGTTATGGCGGAGTAACACTTAATGAAAGTGCTTCATGTGGCAGGGGAAAAATGCAGCTGCAGGGCGTACAGGAGAAAATGGAGGTTACGGTGATATGACGCCTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   696 GenBank   WP_000635824
Name   MbeA_PCN061p2 insolico UniProt ID   _
Length   499 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 499 a.a.        Molecular weight: 55126.54 Da        Isoelectric Point: 8.8190

>WP_000635824.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVRELIDASPYAKKYTSGVLSFAEKDLPPGQR
EKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNMELLTGKRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPSALPKTKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGKGLRAEIESAAAEYQRDAESRIQRAREVCQSGTERKREENQRR
HPRPRPEAVLSHEPAHERHGAYGQPDVADHRPGVCTADSIGRKHSVVAGAADTRQLHDHPGAEEHAGHAV
REEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE
RLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVTRLNVIEAHREQERAAQHQKTLELERSQR
QRTYNGPSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure



No available structure.




Host bacterium

ID   1084 GenBank   NZ_CP006638
Plasmid name   PCN061p2 Incompatibility group   Col440I
Plasmid size   5754 bp Coordinate of oriT [Strand]   1625..1810 [-]
Host baterium   Escherichia coli PCN061

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -