Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100622
Name   oriT_pHS191 in_silico
Organism   Pseudomonas syringae pv. syringae HS191
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP006257 (46795..46920 [+], 126 nt)
oriT length   126 nt
IRs (inverted repeats)      IR1: 28..33, 42..47  (AAAAAG..CTTTTT)
  IR2: 62..73, 77..88  (GTGCCAAAGGGG..CCCCTTTGAAAC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 126 nt

>oriT_pHS191
AAACAGGCACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGTATCCAATCGACGTGCCAAAGGGGATACCCCTTTGAAACCCCGCAGAGCGGGAAAGCGTTGTCGTTGTTGGTTGTTG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   694 GenBank   WP_080348830
Name   Relaxase_pHS191 insolico UniProt ID   _
Length   1249 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1249 a.a.        Molecular weight: 138644.22 Da        Isoelectric Point: 9.2834

>WP_080348830.1 LPD7 domain-containing protein [Pseudomonas syringae]
MLIRVSGYNTGAQEYLEQGNKSGREFTRDELDHRLIIEGQLSVTRAIYESIPDHGQDRYLTFTLSFKEDT
VSPELLKAVTTDFKNFFMHAYKPEEFNLYAEAHLPKMKTVTDRKTGEVIDRKPHIHIIIPRINLLSGNEA
NPVDVYKNHEKYFEAFQEHINQKYGLSSPRENVRADITDAASVLSRYKGDDFYGKNRQFKQQLVKQVIER
GVTSRADFYALVAEHGETRIRNEGKDNEYISVKLPGDAKGTNLKDTIFQDAFFIRRELKKPPLEASVIQE
RLLAWPQRAREIKYVNKATPKFRKQYSQASPEERVRLLAERENNFYRAHGDDYESVHTWQRQRDNQRSSA
EAAGRRTAAPADGLQDLSVSDVADHRQAGPTRSRDGALLLPTDAHVHVGQSQPGGDSGLRSSVPAGGRGR
RAGSTTERGRGGSQPAAVSQETKGTATPAGATGRRRAGAGKSRNTRVRAGRVVPPYAQNPHRVATIADIE
ERGRRLFDPLKRPSDNALVFYRSSSHPQVVDQLPQARAATTTPVQSTAGRRPRSSGKPRPPRQWRPGAVP
PYAKNPHRVATVADIEQRARMLFDPLKRPADKALVFKRASIKALTVNKHASTVAAYFTRQAQHNQIAPAH
RRAIRRIDQQYFALRRAVFSDQRLTRQDKAQLVSVLTFERLKAREQFHKPQPNIEVNLMGSAAIRNLLDD
EKEDPGFSISGARGPGPEGVRDRVKRVMDRFAKQIDPVAASERARDLSAKDLYTRKAKFSQNVHYLDKQT
DKTLFVDTGTTISMRRTGITEAGVSVALQLARERFGSTLTINGTAEFKKLVIEAVAKNGLDVHFTDKAMN
QSLADRRAELDIERGGQSIGPATDLPRHVDDATRDVRDQADRLGVTVPIEALYGQGKTADQVSQALATQL
DTVPEPERIAFVETVAITLAIPERGEPKGDQAFAQWQAQRAQPTADSAATATAETQTGVPTPSDTAPEPV
TPQAAKPALANDPALQSPSELVRLEAQWRRDFPMSETDVRASDTVMGLRGEDHAVWIIATNDKTPEAAAM
LTVYMENDSYREAFKASIVAAYKQVENSPKLIDDLDHLTAMAAQIVNEVEDRLYPAPQAATGQTAPSRSK
VIEGTLIEHGEAPYQHNDDNQMSYFVTLKPEGGKPRTVWGVGLEEAMSDADLKQGDQVRLEDLGTQPVVV
QVIEEDGTVTDKTVNRREWSAQPVAPEREVAETTPKGQAIAAGTPELSSPDEEDGMNMD

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(760-845)

Relaxase/Mobilisation nuclease domain (Relaxase)

(40-255)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 25390..35712

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSYRH_RS27670 (PsyrH_P155) 20727..21254 + 528 WP_162488822 DUF2778 domain-containing protein -
PSYRH_RS27675 21251..21541 + 291 WP_080348826 hypothetical protein -
PSYRH_RS25955 (PsyrH_P160) 21618..22391 - 774 WP_046720401 helix-turn-helix transcriptional regulator -
PSYRH_RS25960 (PsyrH_P165) 22500..23600 + 1101 WP_046720402 polyamine ABC transporter substrate-binding protein -
PSYRH_RS25965 (PsyrH_P170) 23697..23912 + 216 WP_046720403 hypothetical protein -
PSYRH_RS27680 23922..24206 - 285 WP_223123953 helix-turn-helix transcriptional regulator -
PSYRH_RS28695 24245..24382 - 138 WP_162488821 hypothetical protein -
PSYRH_RS25975 (PsyrH_P175) 24513..25046 + 534 WP_046720404 transcription termination/antitermination NusG family protein -
PSYRH_RS25980 (PsyrH_P180) 25135..25377 + 243 WP_046720405 hypothetical protein -
PSYRH_RS25985 (PsyrH_P185) 25390..26079 + 690 WP_046720406 lytic transglycosylase domain-containing protein virB1
PSYRH_RS25990 (PsyrH_P190) 26076..26414 + 339 WP_223227219 conjugal transfer protein -
PSYRH_RS27685 26427..26915 + 489 WP_080348828 VirB3 family type IV secretion system protein virB3
PSYRH_RS25995 (PsyrH_P195) 26842..29316 + 2475 WP_046720432 VirB4 family type IV secretion system protein virb4
PSYRH_RS26000 (PsyrH_P200) 29313..29999 + 687 WP_046720407 P-type DNA transfer protein VirB5 -
PSYRH_RS26005 (PsyrH_P205) 30032..30385 + 354 WP_046720408 hypothetical protein -
PSYRH_RS26010 (PsyrH_P210) 30396..31328 + 933 WP_046720409 type IV secretion system protein virB6
PSYRH_RS26015 (PsyrH_P220) 31739..32527 + 789 WP_046720410 type IV secretion system protein virB8
PSYRH_RS26020 (PsyrH_P225) 32517..33326 + 810 WP_046720411 P-type conjugative transfer protein VirB9 virB9
PSYRH_RS26025 (PsyrH_P230) 33313..34671 + 1359 WP_046720412 TrbI/VirB10 family protein virB10
PSYRH_RS26030 (PsyrH_P235) 34681..35712 + 1032 WP_046720413 P-type DNA transfer ATPase VirB11 virB11
PSYRH_RS26035 (PsyrH_P240) 35722..36078 + 357 WP_010217013 hypothetical protein -
PSYRH_RS26040 (PsyrH_P245) 36214..38622 + 2409 WP_046720414 ATP-binding protein -
PSYRH_RS26045 (PsyrH_P250) 38721..38951 + 231 WP_046720415 hypothetical protein -
PSYRH_RS26050 (PsyrH_P255) 38951..40609 + 1659 WP_046720416 type IV secretory system conjugative DNA transfer family protein -

Host bacterium

ID   1082 GenBank   NZ_CP006257
Plasmid name   pHS191 Incompatibility group   _
Plasmid size   52548 bp Coordinate of oriT [Strand]   46795..46920 [+]
Host baterium   Pseudomonas syringae pv. syringae HS191

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -