Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100615 |
| Name | oriT_pG6809-2  |
| Organism | Enterobacter cloacae |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_032101 (44472..45129 [+], 658 nt) |
| oriT length | 658 nt |
| IRs (inverted repeats) | IR1: 198..207, 211..220 (ATTTGGTGTT..AATACCTAAT) IR2:339..3444, 350..355 (AAAAAA..TTTTTT) IR3: 413..422, 425..434 (GTATTCATGC..GCATGAATAC) |
| Location of nic site | 301..302 |
| Conserved sequence flanking the nic site |
GGTGT|ATAGC |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 658 nt
>oriT_pG6809-2
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTCTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAATCAATGTGTTATTTGGTGTTTTTAATACCTAATTATTACCGAACATTGACGTTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTATATAGCAGGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGAGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACACTATTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAGAATTACATCATTATTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAGGTACATCAAAAATACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAATTGATGTTGCCGG
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTCTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAATCAATGTGTTATTTGGTGTTTTTAATACCTAATTATTACCGAACATTGACGTTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTATATAGCAGGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGAGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACACTATTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAGAATTACATCATTATTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAGGTACATCAAAAATACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAATTGATGTTGCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 687 | GenBank | YP_009328667 |
| Name | TraI_pG6809-2 |
UniProt ID | A0A0P0KT65 |
| Length | 1078 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 1078 a.a. Molecular weight: 120137.33 Da Isoelectric Point: 6.5766
>YP_009328667.1 conjugal transfer protein TraI (plasmid) [Enterobacter cloacae]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0P0KT65 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 11684..22006
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTG25_RS00030 (D727_p7005) | 7529..8962 | - | 1434 | WP_001288432 | DNA cytosine methyltransferase | - |
| HTG25_RS00035 | 9061..9333 | + | 273 | Protein_6 | IS1 family transposase | - |
| HTG25_RS00040 | 9344..9550 | - | 207 | WP_001749967 | hypothetical protein | - |
| HTG25_RS00045 (D727_p7006) | 9555..10202 | - | 648 | WP_015344958 | restriction endonuclease | - |
| HTG25_RS00050 | 10252..10485 | - | 234 | WP_001191790 | hypothetical protein | - |
| HTG25_RS00055 | 10599..10913 | - | 315 | WP_049074891 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTG25_RS00060 | 10910..11254 | - | 345 | WP_049074895 | hypothetical protein | - |
| HTG25_RS00065 | 11270..11641 | - | 372 | WP_011867773 | H-NS family nucleoid-associated regulatory protein | - |
| HTG25_RS00070 (D727_p7008) | 11684..12475 | + | 792 | WP_072105561 | lytic transglycosylase domain-containing protein | virB1 |
| HTG25_RS00075 | 12426..12707 | + | 282 | WP_000440698 | transcriptional repressor KorA | - |
| HTG25_RS00080 | 12717..13010 | + | 294 | WP_001749962 | hypothetical protein | virB2 |
| HTG25_RS00085 | 13058..13375 | + | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
| HTG25_RS00090 (D727_p7009) | 13375..15975 | + | 2601 | WP_042098203 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| HTG25_RS00095 (D727_p7010) | 15993..16706 | + | 714 | WP_013263778 | type IV secretion system protein | virB5 |
| HTG25_RS00100 | 16714..16941 | + | 228 | WP_001749959 | IncN-type entry exclusion lipoprotein EexN | - |
| HTG25_RS00105 (D727_p7011) | 16957..17997 | + | 1041 | WP_001749958 | type IV secretion system protein | virB6 |
| HTG25_RS00110 | 18068..18226 | + | 159 | WP_012561180 | hypothetical protein | - |
| HTG25_RS00115 (D727_p7012) | 18216..18914 | + | 699 | WP_000646594 | type IV secretion system protein | virB8 |
| HTG25_RS00120 (D727_p7013) | 18925..19809 | + | 885 | WP_000735066 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HTG25_RS00125 (D727_p7014) | 19809..20969 | + | 1161 | WP_033547518 | type IV secretion system protein VirB10 | virB10 |
| HTG25_RS00130 (D727_p7015) | 21011..22006 | + | 996 | WP_000128596 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTG25_RS00135 (D727_p7016) | 22006..22538 | + | 533 | Protein_26 | phospholipase D family protein | - |
| HTG25_RS00140 | 22711..23025 | - | 315 | WP_000091613 | hypothetical protein | - |
| HTG25_RS00145 | 23280..23636 | - | 357 | WP_000215515 | cupin domain-containing protein | - |
| HTG25_RS00150 | 23626..24027 | - | 402 | WP_001293886 | DUF86 domain-containing protein | - |
| HTG25_RS00155 | 24024..24314 | - | 291 | WP_001247892 | nucleotidyltransferase | - |
| HTG25_RS00160 (D727_p7018) | 24620..25633 | - | 1014 | WP_000845039 | class 1 integron integrase IntI1 | - |
| HTG25_RS00165 (D727_p7019) | 25800..26600 | + | 801 | WP_013263789 | subclass B1 metallo-beta-lactamase VIM-1 | - |
Host bacterium
| ID | 1075 | GenBank | NC_032101 |
| Plasmid name | pG6809-2 | Incompatibility group | IncN |
| Plasmid size | 58120 bp | Coordinate of oriT [Strand] | 44472..45129 [+] |
| Host baterium | Enterobacter cloacae |
Cargo genes
| Drug resistance gene | blaVIM-1, aac(6')-Il, dfrA1, ant(3'')-Ia |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merC, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |