Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100610
• Name: oriT_pRJ9
• Organism: Staphylococcus aureus
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_025194 (11..249 [+], 239 nt)
• oriT length: 239 nt
• IRs (inverted repeats): IR1: 52..58, 62..68 (TTGGGGA..TCCCCAA) IR2: 94..98, 104..108 (TGGCT..AGCCA)
• Location of nic site: 115..116
• Conserved sequence flanking the nic site: TGCTTG|CCA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 239 nt

>oriT_pRJ9
AGTGGCTAGTAATTAGCCACTATTTTTTCGCCAGAAAAAACCCTAAGGGGATTGGGGAATATCCCCAACAAGCAGGCGTAGCTAACACGCTAGTGGCTAGCGAAGCCAATGCTTGCCAAACCACTTTAACGACTTGATTAAATTTACTTTATCCTATAAAGTAAATTTATATTAAGAAGTTATCTTTCGTAGAAAGCGTGGTGGGATATATGAGCGAACAAAACAGATTTGTGGCTAGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_pRJ9

• ID: 682
• GenBank: YP_009072005
• Name: MobA_pRJ9
• UniProt ID: Q8GPJ1
• Length: 335 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 335 a.a.
• Molecular weight: 38861.76 Da
• Isoelectric Point: 7.9170

>YP_009072005.1 MobA-like protein (plasmid) [Staphylococcus aureus]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQSFKPDEVTPE
QCNQLGLELAEKFAAHHQVAVYTHNDTNHVHNHIVINSINLETGKKFNNNKQALRDLRDFNDQVCRNHDL
SVPEKDTARLRYTQTEKAIADPNIKSTAQYSWKDEIREAIDQSQATNIDELKTHLSQYGIEIERVTSKSI
TYRHLAEDKKVRGRRLGENYNKGGIEDGFERQIQRRQQERESEHDYQPKRRISNRDESTERDTGLTQPDW
DRFAKDTNELERSRKAAESARFINEKARRDREARIQAERETRTIINNDRGHDYEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

• AlphaFold DB: Q8GPJ1

Host bacterium

• ID: 1070
• GenBank: NC_025194
• Plasmid name: pRJ9
• Incompatibility group: _
• Plasmid size: 10406 bp
• Coordinate of oriT [Strand]: 11..249 [+]
• Host baterium: Staphylococcus aureus

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -