Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100604
• Name: oriT_pH1519-7
• Organism: Escherichia coli
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_025178 (2772..2853 [-], 82 nt)
• oriT length: 82 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 82 nt

>oriT_pH1519-7
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_pH1519-7

• ID: 676
• GenBank: YP_009071086
• Name: MobA_pH1519-7
• UniProt ID: A0A075MG93
• Length: 533 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 533 a.a.
• Molecular weight: 59430.16 Da
• Isoelectric Point: 6.3768

>YP_009071086.1 MobA (plasmid) [Escherichia coli]
MSCAGCVWLSGNRGRGMIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYT
SGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYY
DRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDALYHAGEIKGRQDVI
QALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARR
ICKRGCDIKRDENQRRYSPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWR
DALVSWREDTGEPGRNQEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQ
SDRAGNTFAERLRAAATGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEP
VIQRELEIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (73-253)

Protein structure

• AlphaFold DB: A0A075MG93

Host bacterium

• ID: 1064
• GenBank: NC_025178
• Plasmid name: pH1519-7
• Incompatibility group: ColRNAI
• Plasmid size: 7036 bp
• Coordinate of oriT [Strand]: 2772..2853 [-]
• Host baterium: Escherichia coli

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -