Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100602 |
| Name | oriT_pH2291-112  |
| Organism | Escherichia coli |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_025176 (100379..100488 [+], 110 nt) |
| oriT length | 110 nt |
| IRs (inverted repeats) | IR1: 24..31, 35..42 (GTCGGGGC..GCCCTGAC) IR2: 49..65, 72..88 (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC) |
| Location of nic site | 96..97 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 110 nt
>oriT_pH2291-112
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 674 | GenBank | YP_009070937 |
| Name | PutativerelaxaseofpH2291-112 |
UniProt ID | _ |
| Length | 899 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 104010.41 Da Isoelectric Point: 7.4265
>YP_009070937.1 hypothetical protein (plasmid) [Escherichia coli]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 52841..95019
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTC89_RS00305 (D616_p150081) | 47917..48970 | + | 1054 | Protein_58 | type IV pilus biogenesis lipoprotein PilL | - |
| HTC89_RS00310 (D616_p150082) | 48970..49407 | + | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| HTC89_RS00315 (D616_p150083) | 49421..51103 | + | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| HTC89_RS00320 (D616_p150084) | 51096..52391 | + | 1296 | WP_000752772 | type 4b pilus protein PilO2 | - |
| HTC89_RS00325 (D616_p150085) | 52378..52830 | + | 453 | WP_001247334 | type IV pilus biogenesis protein PilP | - |
| HTC89_RS00330 (D616_p150086) | 52841..54394 | + | 1554 | WP_000362202 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTC89_RS00335 (D616_p150087) | 54407..55504 | + | 1098 | WP_001208805 | type II secretion system F family protein | - |
| HTC89_RS00340 (D616_p150088) | 55522..56136 | + | 615 | WP_000959785 | type 4 pilus major pilin | - |
| HTC89_RS00345 (D616_p150089) | 56146..56706 | + | 561 | WP_000014116 | lytic transglycosylase domain-containing protein | virB1 |
| HTC89_RS00350 (D616_p150090) | 56691..57347 | + | 657 | WP_001193553 | prepilin peptidase | - |
| HTC89_RS00355 (D616_p150091) | 57347..58681 | + | 1335 | WP_001496527 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTC89_RS00360 (D616_p150092) | 59159..60421 | + | 1263 | WP_000608644 | IS1380-like element ISEcp1 family transposase | - |
| HTC89_RS00365 (D616_p150093) | 60628..61608 | - | 981 | WP_000019441 | IS5-like element ISKpn26 family transposase | - |
| HTC89_RS00370 (D616_p150094) | 61909..62784 | + | 876 | WP_013188473 | extended-spectrum class A beta-lactamase CTX-M-1 | - |
| HTC89_RS00375 (D616_p150095) | 62831..63175 | - | 345 | Protein_72 | WbuC family cupin fold metalloprotein | - |
| HTC89_RS00635 | 63175..63384 | + | 210 | Protein_73 | hypothetical protein | - |
| HTC89_RS00380 | 64010..64300 | - | 291 | WP_228717225 | prepilin | - |
| HTC89_RS00385 (D616_p150097) | 64284..65438 | + | 1155 | WP_001139957 | site-specific integrase | - |
| HTC89_RS00390 (D616_p150098) | 65589..66413 | + | 825 | WP_001238939 | conjugal transfer protein TraE | traE |
| HTC89_RS00395 (D616_p150099) | 66498..67700 | + | 1203 | WP_000976351 | conjugal transfer protein TraF | - |
| HTC89_RS00400 (D616_p150100) | 67760..68344 | + | 585 | WP_000977522 | histidine phosphatase family protein | - |
| HTC89_RS00405 (D616_p150102) | 68739..69197 | + | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| HTC89_RS00410 (D616_p150103) | 69194..70012 | + | 819 | WP_000646098 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| HTC89_RS00415 (D616_p150104) | 70009..71157 | + | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| HTC89_RS00420 (D616_p150105) | 71154..71444 | + | 291 | WP_001299214 | hypothetical protein | traK |
| HTC89_RS00425 (D616_p150106) | 71459..72010 | + | 552 | WP_000014583 | phospholipase D family protein | - |
| HTC89_RS00430 (D616_p150107) | 72099..75866 | + | 3768 | WP_001141527 | LPD7 domain-containing protein | - |
| HTC89_RS00435 (D616_p150108) | 75884..76231 | + | 348 | WP_001055900 | conjugal transfer protein | traL |
| HTC89_RS00440 (D616_p150109) | 76228..76920 | + | 693 | WP_000138550 | DotI/IcmL family type IV secretion protein | traM |
| HTC89_RS00445 (D616_p150110) | 76931..77914 | + | 984 | WP_001191877 | IncI1-type conjugal transfer protein TraN | traN |
| HTC89_RS00450 (D616_p150111) | 77917..79206 | + | 1290 | WP_001272003 | conjugal transfer protein TraO | traO |
| HTC89_RS00455 (D616_p150112) | 79206..79910 | + | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| HTC89_RS00460 (D616_p150113) | 79910..80437 | + | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| HTC89_RS00465 (D616_p150114) | 80488..80892 | + | 405 | WP_000086958 | IncI1-type conjugal transfer protein TraR | traR |
| HTC89_RS00470 (D616_p150115) | 80956..81144 | + | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| HTC89_RS00475 (D616_p150116) | 81128..81928 | + | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| HTC89_RS00480 (D616_p150117) | 82018..85062 | + | 3045 | WP_021265678 | IncI1-type conjugal transfer protein TraU | traU |
| HTC89_RS00485 (D616_p150119) | 85062..85676 | + | 615 | WP_000337398 | IncI1-type conjugal transfer protein TraV | traV |
| HTC89_RS00490 (D616_p150120) | 85643..86845 | + | 1203 | WP_001189160 | IncI1-type conjugal transfer protein TraW | traW |
| HTC89_RS00495 (D616_p150121) | 86874..87458 | + | 585 | WP_001037987 | IncI1-type conjugal transfer protein TraX | - |
| HTC89_RS00500 (D616_p150122) | 87555..89723 | + | 2169 | WP_021537350 | DotA/TraY family protein | traY |
| HTC89_RS00505 (D616_p150123) | 89773..90447 | + | 675 | WP_223866377 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HTC89_RS00510 (D616_p150124) | 90519..90728 | - | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| HTC89_RS00515 | 91012..91296 | + | 285 | WP_031942457 | hypothetical protein | - |
| HTC89_RS00645 | 91361..91456 | - | 96 | WP_001303310 | DinQ-like type I toxin DqlB | - |
| HTC89_RS00520 (D616_p150127) | 91955..92206 | + | 252 | WP_001291964 | hypothetical protein | - |
| HTC89_RS00525 (D616_p150128) | 92278..92430 | - | 153 | WP_001331364 | Hok/Gef family protein | - |
| HTC89_RS00530 (D616_p150129) | 92722..93930 | + | 1209 | WP_000121273 | IncI1-type conjugal transfer protein TrbA | trbA |
| HTC89_RS00535 (D616_p150130) | 93949..95019 | + | 1071 | WP_000151583 | IncI1-type conjugal transfer protein TrbB | trbB |
| HTC89_RS00540 (D616_p150131) | 95012..97303 | + | 2292 | WP_001289276 | F-type conjugative transfer protein TrbC | - |
Host bacterium
| ID | 1062 | GenBank | NC_025176 |
| Plasmid name | pH2291-112 | Incompatibility group | IncI1 |
| Plasmid size | 112671 bp | Coordinate of oriT [Strand] | 100379..100488 [+] |
| Host baterium | Escherichia coli |
Cargo genes
| Drug resistance gene | sul2, aadA5, blaCTX-M-1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |