Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100601
• Name: oriT_ColicinE1
• Organism: Escherichia coli
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_025157 (93..175 [+], 83 nt)
• oriT length: 83 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 83 nt

>oriT_ColicinE1
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

TraI_ColicinE1

• ID: 673
• GenBank: YP_009070583
• Name: TraI_ColicinE1
• UniProt ID: A0A075M947
• Length: 1763 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 1763 a.a.
• Molecular weight: 193017.09 Da
• Isoelectric Point: 5.9894

>YP_009070583.1 IncF plasmid conjugative transfer DNA-nicking and unwinding protein TraI (plasmid) [Escherichia coli]
MSKGYTFMMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDG
ADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSET
VLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREK
LKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEIK
MVEWMQTLKETGFDIRAYRDAADQRADLRTLTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLART
VGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSV
PRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNMKQDERLS
GELITGRRQLLEGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLIIDSGQRTGTGSALMAMKDA
GVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAVSVKAGEESVAQVSGVREQAMLTQTIRSELKTQGV
LGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVV
RISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVSSVSEDAMTVVVPGRAEPATLPVSDS
PFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSF
TVVSEQIKTRAGETSLETAISHQKSALHTPAQQAIHLALPVVESKKLAFSMVDLLTEAKSFAAEGTGFTE
LGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVMPLMERVPGELMEKLTSGQ
RAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTL
ASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFR
LQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQ
EAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGE
LGKEQVMVPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLISPRE
AVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTREIRPGQEQAEQH
IDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQK
GTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFD
RNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGV
VVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMA
ENKPDLPDGKTEQAVREIAGQERDRAAITEREAALPEGVLREPQRVREAVREIARENLLQERLQQMERDM
VRDLQKEKTLGGD

Protein domains

Predicted by InterproScan.

• single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N) (582-633)

• TrwC relaxase (TrwC) (17-290)

• DNA relaxase TraI 2B/2B-like domain (TraI_2B) (640-719)

• DNA helicase TraI (TraI_C) (1441-1564)

• AAA domain (AAA_30) (976-1163)

Protein structure

No available structure.

Host bacterium

• ID: 1061
• GenBank: NC_025157
• Plasmid name: Colicin E1
• Incompatibility group: ColRNAI
• Plasmid size: 1683 bp
• Coordinate of oriT [Strand]: 93..175 [+]
• Host baterium: Escherichia coli

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -