Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100599 |
| Name | oriT_pCTXM5-1358  |
| Organism | Salmonella enterica subsp. enterica serovar Typhimurium |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_025149 (1959..2133 [+], 175 nt) |
| oriT length | 175 nt |
| IRs (inverted repeats) | 2..11, 15..24 (GTTTCGGGGC..GCCCTGAACC) |
| Location of nic site | 55..56 |
| Conserved sequence flanking the nic site |
CTGG|CTTA |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 175 nt
>oriT_pCTXM5-1358
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGGAAGGAGAAAAAAGGTGGCACCGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGGAAGGAGAAAAAAGGTGGCACCGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 671 | GenBank | YP_009069712 |
| Name | MobA_pCTXM5-1358 |
UniProt ID | I6ZWA6 |
| Length | 515 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 515 a.a. Molecular weight: 57068.61 Da Isoelectric Point: 7.9937
>YP_009069712.1 MobA (plasmid) [Salmonella enterica subsp. enterica serovar Typhimurium]
MPDLSGCGTPFLRKERTMIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKY
TSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGRRLQPY
YDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSGLPKTTQEAAEAITRGLLSLASSGELKSRQDV
TDTLEGAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFYGLEGLRGEIESAAAEYRRDAESRIQRAR
AVCQSGTERKREENQRRHQRPRAEYERGDGEKPAERAADGREDMAHHPDGVSAADRHEREHSVVAGAADN
RQLHASSSAGRYPGENDRPDVGSALSGEQRRPEVSYPAAGDANRSHPLRRDDMDPPETGVAENDRAGKAV
AERIRAATAGLLAKAGRMGERLRGMAEHVWAYATGERDAERARVGLEHAGAEFKRAAAPLVRELNAIELR
QRENEYQKTLELERQPQKTYRGPTL
MPDLSGCGTPFLRKERTMIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKY
TSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGRRLQPY
YDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSGLPKTTQEAAEAITRGLLSLASSGELKSRQDV
TDTLEGAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFYGLEGLRGEIESAAAEYRRDAESRIQRAR
AVCQSGTERKREENQRRHQRPRAEYERGDGEKPAERAADGREDMAHHPDGVSAADRHEREHSVVAGAADN
RQLHASSSAGRYPGENDRPDVGSALSGEQRRPEVSYPAAGDANRSHPLRRDDMDPPETGVAENDRAGKAV
AERIRAATAGLLAKAGRMGERLRGMAEHVWAYATGERDAERARVGLEHAGAEFKRAAAPLVRELNAIELR
QRENEYQKTLELERQPQKTYRGPTL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | I6ZWA6 |
Host bacterium
| ID | 1059 | GenBank | NC_025149 |
| Plasmid name | pCTXM5-1358 | Incompatibility group | Col440II |
| Plasmid size | 8216 bp | Coordinate of oriT [Strand] | 1959..2133 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Typhimurium |
Cargo genes
| Drug resistance gene | blaCTX-M-5 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |