Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100592 |
| Name | oriT_pH1519-88  |
| Organism | Escherichia coli |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_025140 (16568..16677 [-], 110 nt) |
| oriT length | 110 nt |
| IRs (inverted repeats) | IR1: 24..31, 35..42 (GTCGGGGC..GCCCTGAC) IR2: 49..65, 72..88 (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC) |
| Location of nic site | 96..97 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 110 nt
>oriT_pH1519-88
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 664 | GenBank | YP_009068406 |
| Name | PutativerelaxaseofpH1519-88 |
UniProt ID | A0A075MBQ2 |
| Length | 871 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 871 a.a. Molecular weight: 100875.94 Da Isoelectric Point: 7.2859
>YP_009068406.1 hypothetical protein (plasmid) [Escherichia coli]
MTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAA
DMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHV
AVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQ
TFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSHMDGKFLVMDGWDRNREGVQLDSFGPSWCAEKL
MKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHLAERLPEMAREGRLENCQA
IHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWLPVPTDIFRQVTPTERF
RGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFA
QQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAV
SDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYD
DPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYR
DRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
NALVDKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITRPDVDHHREGSER
YYRDYIAANSNDDASLPPPEQDKRWEPPSPG
MTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAA
DMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHV
AVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQ
TFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSHMDGKFLVMDGWDRNREGVQLDSFGPSWCAEKL
MKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHLAERLPEMAREGRLENCQA
IHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWLPVPTDIFRQVTPTERF
RGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFA
QQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAV
SDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYD
DPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYR
DRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
NALVDKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITRPDVDHHREGSER
YYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A075MBQ2 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 22037..59076
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTH92_RS00155 (D616_p140039) | 19753..22044 | - | 2292 | WP_012783931 | F-type conjugative transfer protein TrbC | - |
| HTH92_RS00160 (D616_p140040) | 22037..23107 | - | 1071 | WP_012783932 | IncI1-type conjugal transfer protein TrbB | trbB |
| HTH92_RS00165 (D616_p140041) | 23126..24334 | - | 1209 | WP_001703845 | IncI1-type conjugal transfer protein TrbA | trbA |
| HTH92_RS00170 (D616_p140042) | 24641..25420 | - | 780 | WP_275450201 | protein FinQ | - |
| HTH92_RS00175 (D616_p140043) | 26047..26199 | + | 153 | WP_001387489 | Hok/Gef family protein | - |
| HTH92_RS00180 (D616_p140044) | 26271..26522 | - | 252 | WP_001291964 | hypothetical protein | - |
| HTH92_RS00505 | 27023..27118 | + | 96 | WP_000609148 | DinQ-like type I toxin DqlB | - |
| HTH92_RS00190 | 27183..27467 | - | 285 | WP_001707309 | hypothetical protein | - |
| HTH92_RS00195 (D616_p140049) | 27751..27960 | + | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| HTH92_RS00200 (D616_p140050) | 28032..28694 | - | 663 | WP_012783935 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HTH92_RS00205 (D616_p140051) | 28765..30933 | - | 2169 | WP_015508354 | DotA/TraY family protein | traY |
| HTH92_RS00210 (D616_p140052) | 31030..31614 | - | 585 | WP_001037998 | conjugal transfer protein TraX | - |
| HTH92_RS00215 (D616_p140053) | 31643..32845 | - | 1203 | WP_001690627 | IncI1-type conjugal transfer protein TraW | traW |
| HTH92_RS00220 (D616_p140054) | 32812..33426 | - | 615 | WP_000337399 | IncI1-type conjugal transfer protein TraV | traV |
| HTH92_RS00225 (D616_p140055) | 33426..36470 | - | 3045 | WP_032072194 | IncI1-type conjugal transfer protein TraU | traU |
| HTH92_RS00230 (D616_p140056) | 36560..37360 | - | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| HTH92_RS00235 (D616_p140057) | 37344..37532 | - | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| HTH92_RS00240 (D616_p140058) | 37596..38000 | - | 405 | WP_001422824 | IncI1-type conjugal transfer protein TraR | traR |
| HTH92_RS00245 (D616_p140059) | 38051..38578 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| HTH92_RS00250 (D616_p140060) | 38578..39282 | - | 705 | WP_032072195 | IncI1-type conjugal transfer protein TraP | traP |
| HTH92_RS00255 (D616_p140061) | 39282..40571 | - | 1290 | WP_032072196 | conjugal transfer protein TraO | traO |
| HTH92_RS00260 (D616_p140062) | 40574..41557 | - | 984 | WP_001191879 | IncI1-type conjugal transfer protein TraN | traN |
| HTH92_RS00265 (D616_p140063) | 41568..42260 | - | 693 | WP_000138552 | DotI/IcmL family type IV secretion protein | traM |
| HTH92_RS00270 (D616_p140064) | 42257..42604 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| HTH92_RS00275 (D616_p140065) | 42622..46389 | - | 3768 | WP_032072197 | LPD7 domain-containing protein | - |
| HTH92_RS00280 (D616_p140066) | 46479..47030 | - | 552 | WP_022630639 | phospholipase D family protein | - |
| HTH92_RS00285 (D616_p140067) | 47045..47335 | - | 291 | WP_001299214 | hypothetical protein | traK |
| HTH92_RS00290 (D616_p140068) | 47332..48480 | - | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| HTH92_RS00295 (D616_p140069) | 48477..49295 | - | 819 | WP_032072198 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| HTH92_RS00300 (D616_p140070) | 49292..49750 | - | 459 | WP_022630640 | IncI1-type conjugal transfer lipoprotein TraH | - |
| HTH92_RS00305 (D616_p140072) | 50145..50729 | - | 585 | WP_032072199 | histidine phosphatase family protein | - |
| HTH92_RS00310 (D616_p140073) | 50789..51991 | - | 1203 | WP_000976350 | conjugal transfer protein TraF | - |
| HTH92_RS00315 (D616_p140074) | 52077..52901 | - | 825 | WP_021565538 | conjugal transfer protein TraE | traE |
| HTH92_RS00320 | 53109..53234 | + | 126 | Protein_62 | transposase | - |
| HTH92_RS00325 (D616_p140075) | 53291..54583 | - | 1293 | WP_001350014 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTH92_RS00330 (D616_p140076) | 54583..55239 | - | 657 | WP_001193549 | A24 family peptidase | - |
| HTH92_RS00335 | 55224..55784 | - | 561 | WP_072105544 | lytic transglycosylase domain-containing protein | virB1 |
| HTH92_RS00340 (D616_p140077) | 55794..56408 | - | 615 | WP_032072200 | type 4 pilus major pilin | - |
| HTH92_RS00345 (D616_p140078) | 56425..57510 | - | 1086 | WP_001208802 | type II secretion system F family protein | - |
| HTH92_RS00350 (D616_p140079) | 57523..59076 | - | 1554 | WP_000362206 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTH92_RS00355 (D616_p140080) | 59087..59539 | - | 453 | WP_001247337 | type IV pilus biogenesis protein PilP | - |
| HTH92_RS00360 (D616_p140081) | 59526..60821 | - | 1296 | WP_000752780 | type 4b pilus protein PilO2 | - |
| HTH92_RS00365 (D616_p140082) | 60814..62496 | - | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| HTH92_RS00370 (D616_p140083) | 62510..62947 | - | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| HTH92_RS00375 (D616_p140084) | 62947..64014 | - | 1068 | WP_032072201 | type IV pilus biogenesis lipoprotein PilL | - |
Host bacterium
| ID | 1052 | GenBank | NC_025140 |
| Plasmid name | pH1519-88 | Incompatibility group | IncI1 |
| Plasmid size | 88678 bp | Coordinate of oriT [Strand] | 16568..16677 [-] |
| Host baterium | Escherichia coli |
Cargo genes
| Drug resistance gene | blaCTX-M-1, blaTEM-210 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrVA2 |