Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100591
Name   oriT_pH2291-144 in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_025139 (139720..140009 [+], 290 nt)
oriT length   290 nt
IRs (inverted repeats)      225..232, 235..242 n  (GCAAAAAC..GTTTTTGC)
Location of nic site      251..252
Conserved sequence flanking the
  nic site    		  GTGGGGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 290 nt

>oriT_pH2291-144
CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAACGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACACTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   663 GenBank   YP_009068318
Name   TraI_pH2291-144 insolico UniProt ID   Q84A21
Length   1756 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192202.14 Da        Isoelectric Point: 5.9371

>YP_009068318.1 IncF plasmid conjugative transfer DNA-nicking and unwinding protein TraI (plasmid) [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMVEWMQT
LKETGFDIRAYRDAADQRADLRTLTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNMKQDERLSGELITGR
RQLLEGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLIIDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAVSVKAGEESVAQVSGVREQAMLTQTIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVSSVSEDAMTVVVPGRAEPATLPVSDSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KTRAGETSLETAISHQKSALHTPAQQAIHLALPVVESKKLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVMPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTREIRPGQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRAAITEREAALPEGVLREPQRVREAVREIARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(10-283)

AAA domain (AAA_30)

(969-1156)

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 114169..140571

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTC79_RS00600 113535..113762 + 228 WP_000450532 toxin-antitoxin system antitoxin VapB -
HTC79_RS00605 113762..114160 + 399 WP_000911324 type II toxin-antitoxin system VapC family toxin -
HTC79_RS00610 (D616_p139160) 114169..116322 - 2154 WP_000009350 type IV conjugative transfer system coupling protein TraD virb4
HTC79_RS00615 (D616_p139162) 116575..117306 - 732 WP_000850422 conjugal transfer complement resistance protein TraT -
HTC79_RS00620 (D616_p139163) 117331..117852 - 522 WP_000632670 conjugal transfer entry exclusion protein TraS -
HTC79_RS00625 (D616_p139164) 117885..120701 - 2817 WP_001007054 conjugal transfer mating-pair stabilization protein TraG traG
HTC79_RS00630 (D616_p139165) 120698..122071 - 1374 WP_021553026 conjugal transfer pilus assembly protein TraH traH
HTC79_RS00635 (D616_p139167) 122058..122452 - 395 Protein_131 F-type conjugal transfer protein TrbF -
HTC79_RS00640 (D616_p139168) 122439..122780 - 342 WP_001443031 P-type conjugative transfer protein TrbJ -
HTC79_RS00645 (D616_p139169) 122710..123255 - 546 WP_000059850 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HTC79_RS00650 (D616_p139170) 123242..123526 - 285 WP_000624109 type-F conjugative transfer system pilin chaperone TraQ -
HTC79_RS00655 (D616_p139171) 123607..123921 + 315 WP_000415567 hypothetical protein -
HTC79_RS00660 (D616_p139172) 123923..124270 - 348 WP_001287913 conjugal transfer protein TrbA -
HTC79_RS00665 (D616_p139173) 124286..125029 - 744 WP_001030367 type-F conjugative transfer system pilin assembly protein TraF traF
HTC79_RS00670 (D616_p139174) 125022..125279 - 258 WP_000864318 conjugal transfer protein TrbE -
HTC79_RS00675 (D616_p139175) 125306..127114 - 1809 WP_000821847 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HTC79_RS00680 (D616_p139176) 127111..127749 - 639 WP_011117354 type-F conjugative transfer system pilin assembly protein TrbC trbC
HTC79_RS00685 (D616_p139177) 127758..128750 - 993 WP_000830187 conjugal transfer pilus assembly protein TraU traU
HTC79_RS00690 (D616_p139178) 128747..129379 - 633 WP_032072184 type-F conjugative transfer system protein TraW traW
HTC79_RS00695 (D616_p139179) 129376..129762 - 387 WP_000097319 type-F conjugative transfer system protein TrbI -
HTC79_RS00700 (D616_p139180) 129759..132386 - 2628 WP_001064247 type IV secretion system protein TraC virb4
HTC79_RS00705 (D616_p139182) 132546..132767 - 222 WP_001278689 conjugal transfer protein TraR -
HTC79_RS00710 (D616_p139183) 132902..133417 - 516 WP_000809841 type IV conjugative transfer system lipoprotein TraV traV
HTC79_RS00715 (D616_p139184) 133414..133665 - 252 WP_001038342 conjugal transfer protein TrbG -
HTC79_RS00720 (D616_p139185) 133677..133868 - 192 WP_032072783 conjugal transfer protein TrbD -
HTC79_RS00725 (D616_p139186) 133861..134451 - 591 WP_000002792 conjugal transfer pilus-stabilizing protein TraP -
HTC79_RS00730 (D616_p139187) 134441..135868 - 1428 WP_000146692 F-type conjugal transfer pilus assembly protein TraB traB
HTC79_RS00735 (D616_p139188) 135868..136596 - 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
HTC79_RS00740 (D616_p139189) 136583..137149 - 567 WP_000399792 type IV conjugative transfer system protein TraE traE
HTC79_RS00745 (D616_p139190) 137171..137482 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
HTC79_RS00750 (D616_p139191) 137497..137862 - 366 WP_000994779 type IV conjugative transfer system pilin TraA -
HTC79_RS00755 (D616_p139192) 137895..138296 - 402 WP_001369361 conjugal transfer relaxosome DNA-bindin protein TraY -
HTC79_RS00760 (D616_p139193) 138389..139078 - 690 WP_000283385 conjugal transfer transcriptional regulator TraJ -
HTC79_RS00765 (D616_p139194) 139265..139648 - 384 WP_001151524 conjugal transfer relaxosome DNA-binding protein TraM -
HTC79_RS00770 (D616_p139195) 139969..140571 + 603 WP_000243709 transglycosylase SLT domain-containing protein virB1
HTC79_RS00775 (D616_p139198) 140868..141689 - 822 WP_001234469 DUF932 domain-containing protein -
HTC79_RS00780 (D616_p139199) 141810..142097 - 288 WP_000107535 hypothetical protein -
HTC79_RS00785 (D616_p139200) 142122..142328 - 207 WP_000547939 hypothetical protein -
HTC79_RS00860 142398..142571 + 174 Protein_162 hypothetical protein -
HTC79_RS00865 142569..142799 - 231 WP_071587244 hypothetical protein -
HTC79_RS00790 (D616_p139204) 143019..143144 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
HTC79_RS00870 143086..143235 - 150 Protein_165 plasmid maintenance protein Mok -
HTC79_RS00795 143221..143445 + 225 WP_001311047 protein sok -
HTC79_RS00800 (D616_p139206) 143414..144176 - 763 Protein_167 plasmid SOS inhibition protein A -
HTC79_RS00805 (D616_p139207) 144173..144607 - 435 WP_000845901 conjugation system SOS inhibitor PsiB -

Host bacterium

ID   1051 GenBank   NC_025139
Plasmid name   pH2291-144 Incompatibility group   IncFIB
Plasmid size   144925 bp Coordinate of oriT [Strand]   139720..140009 [+]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   sitABCD, tet(A), blaTEM-1B, sul1, qacE, ant(3'')-Ia, dfrA1
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -