Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100590 |
| Name | oriT_pFOX-7a  |
| Organism | Klebsiella pneumoniae |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_025134 (30590..30695 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pFOX-7a
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGGCCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 662 | GenBank | YP_009067888 |
| Name | MobA_pFOX-7a |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>YP_009067888.1 MobA (plasmid) [Klebsiella pneumoniae]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 8638..27794
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HXG90_RS00020 (D647_p51005) | 4174..4731 | - | 558 | WP_001217881 | recombinase family protein | - |
| HXG90_RS00025 (D647_p51006) | 4893..7898 | + | 3006 | WP_176455437 | Tn3-like element Tn3 family transposase | - |
| HXG90_RS00030 (D647_p51007) | 7982..8635 | - | 654 | WP_004206935 | hypothetical protein | - |
| HXG90_RS00035 (D647_p51008) | 8638..10818 | - | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| HXG90_RS00040 (D647_p51009) | 10811..11461 | - | 651 | WP_004187488 | hypothetical protein | - |
| HXG90_RS00045 (D647_p51010) | 11458..12666 | - | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| HXG90_RS00050 (D647_p51011) | 12663..15713 | - | 3051 | WP_011154474 | conjugative transfer protein | traU |
| HXG90_RS00055 | 15710..16204 | - | 495 | WP_004187480 | hypothetical protein | - |
| HXG90_RS00060 (D647_p51012) | 16250..16639 | - | 390 | WP_011154472 | DUF6750 family protein | traR |
| HXG90_RS00065 (D647_p51013) | 16656..17186 | - | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| HXG90_RS00070 (D647_p51014) | 17210..17914 | - | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| HXG90_RS00075 (D647_p51015) | 17926..19275 | - | 1350 | WP_032072103 | conjugal transfer protein TraO | traO |
| HXG90_RS00080 (D647_p51016) | 19287..20438 | - | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| HXG90_RS00085 (D647_p51017) | 20447..21229 | - | 783 | WP_004206929 | DotI/IcmL family type IV secretion protein | traM |
| HXG90_RS00090 | 21228..21638 | + | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| HXG90_RS00095 | 21625..21822 | + | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| HXG90_RS00100 (D647_p51018) | 21823..22335 | - | 513 | WP_011091071 | hypothetical protein | traL |
| HXG90_RS00105 (D647_p51019) | 22301..23980 | - | 1680 | WP_032072104 | LPD7 domain-containing protein | - |
| HXG90_RS00110 | 23977..25566 | - | 1590 | WP_176455438 | toprim domain-containing protein | - |
| HXG90_RS00115 (D647_p51020) | 25591..25851 | - | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| HXG90_RS00120 (D647_p51021) | 25841..27004 | - | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| HXG90_RS00125 (D647_p51022) | 27015..27794 | - | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| HXG90_RS00130 (D647_p51023) | 27791..28291 | - | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| HXG90_RS00135 (D647_p51024) | 28305..30284 | - | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| HXG90_RS00555 | 30271..30834 | - | 564 | WP_020277892 | plasmid mobilization protein MobA | - |
| HXG90_RS00145 (D647_p51026) | 30833..31228 | + | 396 | WP_019725163 | hypothetical protein | - |
| HXG90_RS00150 | 31730..32266 | - | 537 | WP_004187332 | hypothetical protein | - |
| HXG90_RS00155 | 32399..32710 | - | 312 | WP_004187333 | hypothetical protein | - |
Host bacterium
| ID | 1050 | GenBank | NC_025134 |
| Plasmid name | pFOX-7a | Incompatibility group | IncL/M |
| Plasmid size | 90439 bp | Coordinate of oriT [Strand] | 30590..30695 [+] |
| Host baterium | Klebsiella pneumoniae |
Cargo genes
| Drug resistance gene | blaTEM-1A, aac(6')-Ib, blaFOX-7, qacE, sul1 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merC, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA8 |