Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100588
Name   oriT_pM131_NDM1 in_silico
Organism   Acinetobacter sp. M131
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   NC_025116 (8860..8897 [+], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      8..17, 21..30  (CATAAGGGAA..TTCCCTTATG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 38 nt

>oriT_pM131_NDM1
AGGGATTCATAAGGGAATTATTCCCTTATGTGGGGCTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   660 GenBank   YP_009067138
Name   MobA_pM131_NDM1 insolico UniProt ID   V9M5A3
Length   970 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 970 a.a.        Molecular weight: 110764.85 Da        Isoelectric Point: 8.1822

>YP_009067138.1 putative MobA or MobL protein (plasmid) [Acinetobacter sp. M131]
MAIYHLSVKTGRQYGSTGTRGANHYAYINRDGKYKRDDLENIKSGNLPSWATDAKHFWKSADKNERINGR
VYTELEISLPRELNKEQREELVEKFVEKTLGENFTYSYAIHSPLASDGQQNPHVHLMFSERKLDGIERDE
VKHFKRYNPVNPEKGGAGKDRYFSSKMFVVDTRLEWANHVNDFCENLGIDARIDHRSFKEQGIELSSQNF
RADYVSSHKYHINENIKQVRHQNGEIIIERPSEAIKALTSNQSVFSVRELERFLMAHTDGEEQYLKAYES
VITCPDMATLYGKDKVVFSSKELVGIEESISTFIYNANEESRIQKPFNLVEKLPLNAVRVAETRTFNSEQ
EKAFYTLTSTDRITLLNGSAGTGKSYVLSAVSEAYKESDYQVYGIALQAITAKAIANDCDIPSSTIASFL
SKYDSGNLEINNKTVLILDEAGMVGSRDMQRLLMLVEKHDAQIKLVGDSYQLSAVSAGHVFANIQENLDK
KNIASLEDIQRQKHAKESDKMIQASKYLSKHEVGKAIDIYKSINMVNEYESHDLALAKTVKSWSEDSSES
KLMLAHSNSDVNELNRMARALLIKQGKLNPESVSVKNYQGDLDISIGEKIVFKQNNSQMKVSNGELAKVI
GFEKDKFNNPKAMMVQMESDSRIVRVDLKEYDQFKHGYANTIHSSQGMTVDSAYIVASENMNANLTYVAL
TRHKQNIQINYSALTFATNEKETVKTPDGKVSYDHNYKPVEREVTAFENFKKVLGRSETKEFSTDFTLVE
SKDNLMKSYLNDHRLHLENKRELFGINSKLYSLTSENKTFTKEEIVSEVRASLKSKALLGDEIVKFNGNM
NIAKGELVKLTNDLEIKTGLFKKVTFEKGTELKVIDAYKVKDKPVMKARIKNEEYEIPFNKLNLEYSDKY
FNEFKDQSKARFDSRHKTEVYTEFREQLLKQKANAPKDNLNAPNQRIKNPNTPNRNTPKY

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(43-208)

AAA domain (AAA_30)

(346-538)

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(661-709)


  Protein structure


Source ID Structure
AlphaFold DB V9M5A3


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 31314..44609

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE95_RS00150 (MA28_p29) 27192..27839 - 648 WP_004993315 recombinase family protein -
HPE95_RS00155 (MA28_p30) 27991..28215 + 225 WP_015056389 hypothetical protein -
HPE95_RS00160 (MA28_p31) 28399..30111 + 1713 WP_015060705 zeta toxin family protein -
HPE95_RS00165 (MA28_p32) 30108..30590 - 483 WP_015060706 thermonuclease family protein -
HPE95_RS00170 30790..31251 - 462 WP_015060707 hypothetical protein -
HPE95_RS00175 (MA28_p33) 31314..31955 - 642 WP_004999358 type IV secretion system protein virB5
HPE95_RS00180 (MA28_p34) 31952..34663 - 2712 WP_015060708 VirB4 virb4
HPE95_RS00185 (MA28_p35) 34675..34968 - 294 WP_017480461 TrbC/VirB2 family protein virB2
HPE95_RS00190 (MA28_p36) 34979..35689 - 711 WP_017480462 lytic transglycosylase domain-containing protein -
HPE95_RS00195 (MA28_p37) 35734..35934 - 201 WP_004999346 hypothetical protein -
HPE95_RS00200 (MA28_p38) 35953..37470 - 1518 WP_015060710 type IV secretion system DNA-binding domain-containing protein virb4
HPE95_RS00205 (MA28_p39) 37467..38720 - 1254 WP_015060711 lytic transglycosylase domain-containing protein -
HPE95_RS00210 (MA28_p40) 38796..39248 - 453 WP_004999340 hypothetical protein -
HPE95_RS00215 (MA28_p41) 39252..39581 - 330 WP_004999338 hypothetical protein -
HPE95_RS00220 (MA28_p42) 39550..40587 - 1038 WP_015060713 P-type DNA transfer ATPase VirB11 virB11
HPE95_RS00225 (MA28_p43) 40603..41982 - 1380 WP_005000433 TrbI/VirB10 family protein virB10
HPE95_RS00230 (MA28_p44) 41984..42721 - 738 WP_005000435 TrbG/VirB9 family P-type conjugative transfer protein virB9
HPE95_RS00235 (MA28_p45) 42736..43524 - 789 WP_005000436 VirB8/TrbF family protein virB8
HPE95_RS00240 (MA28_p46) 43524..44609 - 1086 WP_005000437 type IV secretion system protein virB6
HPE95_RS00245 (MA28_p47) 44617..45036 - 420 WP_005000439 hypothetical protein -
HPE95_RS00250 (MA28_p48) 45023..45394 - 372 WP_005000442 hypothetical protein -
HPE95_RS00255 (MA28_p50) 47016..47255 + 240 WP_005000443 hypothetical protein -

Host bacterium

ID   1048 GenBank   NC_025116
Plasmid name   pM131_NDM1 Incompatibility group   _
Plasmid size   47271 bp Coordinate of oriT [Strand]   8860..8897 [+]
Host baterium   Acinetobacter sp. M131

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -