Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100584
• Name: oriT_pUB2380
• Organism: Escherichia coli
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_025013 (802..884 [-], 83 nt)
• oriT length: 83 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 83 nt

>oriT_pUB2380
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

PutativerelaxaseofpUB2380

• ID: 656
• GenBank: YP_009062853
• Name: PutativerelaxaseofpUB2380
• UniProt ID: Q51619
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57837.38 Da
• Isoelectric Point: 6.0811

>YP_009062853.1 putative mob3 protein (plasmid) [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGIPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLLN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRR
YSPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRN
QEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGATLERADRTLEPVIQRELEIREERLIQE
REHVLSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

• AlphaFold DB: Q51619

Host bacterium

• ID: 1044
• GenBank: NC_025013
• Plasmid name: pUB2380
• Incompatibility group: ColRNAI
• Plasmid size: 8561 bp
• Coordinate of oriT [Strand]: 802..884 [-]
• Host baterium: Escherichia coli

Cargo genes

• Drug resistance gene: aph(3')-Ia
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -