Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100583
• Name: oriT_pECL18
• Organism: Enterobacter cloacae
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_025004 (1..51 [-], 51 nt)
• oriT length: 51 nt
• IRs (inverted repeats): 1..10, 14..23 (GTGTCGGGGC..GCCATGACCC)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: predicted by the oriTfinder

oriT sequence

• Length: 51 nt

>oriT_pECL18
GTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

MbeA_pECL18

• ID: 655
• GenBank: YP_009062823
• Name: MbeA_pECL18
• UniProt ID: O69424
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 58116.64 Da
• Isoelectric Point: 5.6403

>YP_009062823.1 MbeA (plasmid) [Enterobacter cloacae]
MIVKFHARGKGGGSGPVDYLLGRESNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVN
HHYWLHDPNAPENRRILTLPDNLPETKQALAESVTRWIDALYHVGEIKGRQDVIQALTEAGIEVVRVTRS
SISIADPNGGRNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRR
YSPVHSLDREIAGKTPGRGERGDDAAQEGCVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRN
QEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIQE
REHVLSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

• AlphaFold DB: O69424

Host bacterium

• ID: 1043
• GenBank: NC_025004
• Plasmid name: pECL18
• Incompatibility group: ColRNAI
• Plasmid size: 5571 bp
• Coordinate of oriT [Strand]: 1..51 [-]
• Host baterium: Enterobacter cloacae

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -