Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100582
Name   oriT_pCY-MdT in_silico
Organism   Enterobacter cloacae
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_025003 (1969..2153 [+], 185 nt)
oriT length   185 nt
IRs (inverted repeats)      14..21, 25..32  (TTCGGGGC..GCCCTGAA)
Location of nic site      65..66
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 185 nt

>oriT_pCY-MdT
GGTTTTAGCAGGTTTCGGGGCGCAGCCCTGAACCAGTCACCTAGCGCTAGCGGAGTGTATACTGGCTTAGTATGTTACGGCGAGATGGAGATTCAGGAAAGTGCTTCATGTGAGAGGGGGAAAATGCTGCTGCAGGGAGTAGGTGCGAAATGCCGGTGTCGGTGACATGACGCCTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   654 GenBank   YP_009062813
Name   MobA_pCY-MdT insolico UniProt ID   A0A076UB37
Length   516 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 516 a.a.        Molecular weight: 57109.87 Da        Isoelectric Point: 9.1312

>YP_009062813.1 mobilization protein MobA (plasmid) [Enterobacter cloacae]
MPDSSGCGMPCWKRVQTMIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVRELIDASPYAKKY
TSGVLSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNMELLTGRRLQPY
YDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPKTKQEAAQAITRGLLALASSGELKTRQDV
TEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGKGLRAEIESAAAEYRRDAESRIQRAR
EVCQSGTERKREENQRRHPRPRPEAVLSHEPAHERHGAYGQPDVADHRPGVYTADSIGRKHSMVAGAADT
RQLHDHPGAEEHAGHAVREEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAER
IRAATAGLLEKAGRVGERLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVTRLNVIEAHREQ
ERAAQHQKALELERSQRQRTYNGPSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(74-255)


  Protein structure


Source ID Structure
AlphaFold DB A0A076UB37


Host bacterium

ID   1042 GenBank   NC_025003
Plasmid name   pCY-MdT Incompatibility group   Col440II
Plasmid size   5933 bp Coordinate of oriT [Strand]   1969..2153 [+]
Host baterium   Enterobacter cloacae

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -