Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100561
• Name: oriT_pACN001-E
• Organism: Escherichia coli ACN001
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_023328 (258..334 [-], 77 nt)
• oriT length: 77 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCATGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 77 nt

>oriT_pACN001-E
GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAATCATTTGGCATCAGTG

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_pACN001-E

• ID: 633
• GenBank: YP_008998692
• Name: MobA_pACN001-E
• UniProt ID: A0A140WYX1
• Length: 451 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 451 a.a.
• Molecular weight: 50147.36 Da
• Isoelectric Point: 7.8155

>YP_008998692.1 MobA protein (plasmid) [Escherichia coli ACN001]
MPGLEKNQYSILWVEHQDKGRLELNFVIPNMELASGKRLQPYYDRADRPRINAWQTLVNHHYGLHDPNAP
ENRRTLVTPNNLPKAKQEAAEAITRGLEALYHAGALKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGR
NLRLKGALYEQSFTNGDRLREETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRRYQRTHSSGERT
TGDAEQCRERAVQRHRERTGPAAGRAALEAGQRADTVPVADGALRHGRDFSGVMGADSVAGKPDCGEHPG
YQGPGRDAGETPGKNMGCDVSRGQEREISGVAVRGEGGKQLDGGEEERSKTGQGVSEYDRAGNALTERLR
AIAAGLYAAAERLGERLHGFAAYVRADTTGERATEGTVRRLESAGAEISRAGGPLESVIYHEQQIRDEQE
RQARLERERARSWHEEQEREARVWRGPSMGM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (3-158)

Protein structure

• AlphaFold DB: A0A140WYX1

Host bacterium

• ID: 1021
• GenBank: NC_023328
• Plasmid name: pACN001-E
• Incompatibility group: ColRNAI
• Plasmid size: 6822 bp
• Coordinate of oriT [Strand]: 258..334 [-]
• Host baterium: Escherichia coli ACN001

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -