Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100560
Name   oriT_pACN001-B in_silico
Organism   Escherichia coli ACN001
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_023327 (109227..109688 [-], 462 nt)
oriT length   462 nt
IRs (inverted repeats)      142..151, 154..163  (ATAGGGTCGT..ACGACCCTAT)
 231..238, 241..248  (GCAAAAAC..GTTTTTGC)
Location of nic site      257..258
Conserved sequence flanking the
  nic site    		  GTGGGGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 462 nt

>oriT_pACN001-B
AAGAAGAGCACCCCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGCTAGGGGCGGCCCTAATATATCCAAATGTTTTTCATGAAAATTGTCAGCACTGACCCTAACAAGGGTCGTCATAGGGTCGCTATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAAATTAAAATACATTATTTAGAACATAAACTAATGATTTAAAAAACAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTACTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAATGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   632 GenBank   YP_008998619
Name   TraI_pACN001-B insolico UniProt ID   A0A140WYN1
Length   1074 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1074 a.a.        Molecular weight: 117013.58 Da        Isoelectric Point: 5.8538

>YP_008998619.1 conjugal transfer nickase/helicase TraI (plasmid) [Escherichia coli ACN001]
MTAQSNSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSED
AMTVVVPGRAEPASLPVSDSPFMALKLENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVR
LYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKTGLHTPAQQAIHLALPVVESKNLAFS
MVDLLTEAKSFAAEGTSFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEA
VTPLMERVPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGL
GPTHRAVGEMRSAGVDAQTLASFLHDTQLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIAAGGGR
AVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQV
PRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHL
NEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKD
DGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVT
LSDGQQTRVIRPGQERAEQHIGLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHV
QVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDVVAGRAVLRQAGLAGGDSPA
RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESL
LADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPRTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQA
EEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAV
REVARENLLQERLQQMERDMVRDL

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(2-39)

AAA domain (AAA_30)

(296-483)

DNA helicase TraI (TraI_C)

(761-884)


  Protein structure


Source ID Structure
AlphaFold DB A0A140WYN1


Host bacterium

ID   1020 GenBank   NC_023327
Plasmid name   pACN001-B Incompatibility group   IncFIB
Plasmid size   168543 bp Coordinate of oriT [Strand]   109227..109688 [-]
Host baterium   Escherichia coli ACN001

Cargo genes

Drug resistance gene   sitABCD
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -