Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100559
Name   oriT_pACN001-F in_silico
Organism   Escherichia coli ACN001
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_023326 (86731..86840 [+], 110 nt)
oriT length   110 nt
IRs (inverted repeats)      IR1: 24..31, 35..42  (GTCGGGGC..GCCCTGAC)
 IR2: 49..65, 72..88  (GTAATTGTAATAGCGTC..GACGGTATTACAATTAC)
Location of nic site      96..97
Conserved sequence flanking the
  nic site    		  CATCCTG|T
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 110 nt

>oriT_pACN001-F
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTCAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   631 GenBank   YP_008998441
Name   Nuclease_pACN001-F insolico UniProt ID   A0A140WZ89
Length   900 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 900 a.a.        Molecular weight: 104037.65 Da        Isoelectric Point: 7.3321

>YP_008998441.1 relaxase/mobilization nuclease domain protein (plasmid) [Escherichia coli ACN001]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVDFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVDAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHCTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLNGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRIVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
YNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKLWEPPSPMR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A140WZ89


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 59079..80391

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7C79_RS00330 (J444_pF80) 54141..55208 + 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -
G7C79_RS00335 (J444_pF81) 55208..55645 + 438 WP_000539807 type IV pilus biogenesis protein PilM -
G7C79_RS00340 (J444_pF82) 55659..57341 + 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
G7C79_RS00345 (J444_pF83) 57334..58629 + 1296 WP_024129991 type 4b pilus protein PilO2 -
G7C79_RS00350 (J444_pF84) 58616..59068 + 453 WP_001247336 type IV pilus biogenesis protein PilP -
G7C79_RS00355 (J444_pF85) 59079..60632 + 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
G7C79_RS00360 (J444_pF86) 60645..61742 + 1098 WP_001208805 type II secretion system F family protein -
G7C79_RS00365 (J444_pF87) 61760..62374 + 615 WP_000959785 type 4 pilus major pilin -
G7C79_RS00370 62384..62944 + 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
G7C79_RS00375 (J444_pF89) 62929..63585 + 657 WP_001193553 prepilin peptidase -
G7C79_RS00380 (J444_pF90) 63585..64919 + 1335 WP_001496527 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
G7C79_RS00540 64916..65215 - 300 WP_223873359 pilus assembly protein PilV -
G7C79_RS00390 (J444_pF91) 65199..66353 + 1155 WP_001139957 site-specific integrase -
G7C79_RS00395 (J444_pF92) 66504..67328 + 825 WP_001238939 conjugal transfer protein TraE traE
G7C79_RS00400 (J444_pF94) 67413..68615 + 1203 WP_000976351 conjugal transfer protein TraF -
G7C79_RS00405 (J444_pF95) 68675..69259 + 585 WP_000977522 histidine phosphatase family protein -
G7C79_RS00410 (J444_pF96) 69385..70623 + 1239 Protein_82 IncI1-type conjugal transfer protein TraU -
G7C79_RS00415 (J444_pF98) 70623..71237 + 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
G7C79_RS00420 (J444_pF99) 71204..72406 + 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
G7C79_RS00425 (J444_pF100) 72435..73019 + 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
G7C79_RS00430 (J444_pF101) 73116..75284 + 2169 WP_000698360 IncI1-type conjugal transfer membrane protein TraY traY
G7C79_RS00435 (J444_pF102) 75360..75974 + 615 WP_000578649 plasmid IncI1-type surface exclusion protein ExcA -
G7C79_RS00440 (J444_pF103) 76072..76281 + 210 WP_001140545 hemolysin expression modulator Hha -
G7C79_RS00445 (J444_pF104) 76382..76666 + 285 WP_024129996 hypothetical protein -
G7C79_RS00545 76731..76826 - 96 WP_000609148 DinQ-like type I toxin DqlB -
G7C79_RS00455 (J444_pF106) 77327..77578 + 252 WP_001535703 hypothetical protein -
G7C79_RS00460 (J444_pF107) 77650..77802 - 153 WP_001331364 Hok/Gef family protein -
G7C79_RS00465 (J444_pF108) 78094..79302 + 1209 WP_001535704 IncI1-type conjugal transfer protein TrbA trbA
G7C79_RS00470 (J444_pF109) 79321..80391 + 1071 WP_001535705 IncI1-type conjugal transfer protein TrbB trbB
G7C79_RS00475 (J444_pF110) 80384..82675 + 2292 WP_001289272 F-type conjugative transfer protein TrbC -
G7C79_RS00480 (J444_pF111) 82748..83566 - 819 WP_000106196 DUF2971 domain-containing protein -

Host bacterium

ID   1019 GenBank   NC_023326
Plasmid name   pACN001-F Incompatibility group   IncI1
Plasmid size   92447 bp Coordinate of oriT [Strand]   86731..86840 [+]
Host baterium   Escherichia coli ACN001

Cargo genes

Drug resistance gene   blaTEM-1B, sul2, aph(3'')-Ib, aph(6)-Id, dfrA1, ant(3'')-Ia, qacE, sul1
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -