Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100558
• Name: oriT_pACN001-C
• Organism: Escherichia coli ACN001
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_023324 (5494..5576 [+], 83 nt)
• oriT length: 83 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCATGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 83 nt

>oriT_pACN001-C
GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGTGCAGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

MbeA_pACN001-C

• ID: 630
• GenBank: YP_008998316
• Name: MbeA_pACN001-C
• UniProt ID: A0A140WYV4
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57979.36 Da
• Isoelectric Point: 5.8991

>YP_008998316.1 DNA relaxase MbeA (plasmid) [Escherichia coli ACN001]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGRNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRVQEARRVCQRGCDLKRDENQRR
YSPVHSLDREIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYHEWRDALVSWRTDTDEPGRN
QEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIQE
REHVLSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

• AlphaFold DB: A0A140WYV4

Host bacterium

• ID: 1018
• GenBank: NC_023324
• Plasmid name: pACN001-C
• Incompatibility group: ColRNAI
• Plasmid size: 5784 bp
• Coordinate of oriT [Strand]: 5494..5576 [+]
• Host baterium: Escherichia coli ACN001

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -