Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100553 |
| Name | oriT_pE71T  |
| Organism | Klebsiella pneumoniae |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_023027 (35104..35209 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pE71T
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 625 | GenBank | YP_008888837 |
| Name | MobA_pE71T |
UniProt ID | R9R6T9 |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>YP_008888837.1 MobA (plasmid) [Klebsiella pneumoniae]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R9R6T9 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 38005..57290
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTA72_RS00280 (KPE71T_00300) | 33089..33400 | + | 312 | WP_004187333 | hypothetical protein | - |
| HTA72_RS00285 (KPE71T_00305) | 33533..34069 | + | 537 | WP_004187332 | hypothetical protein | - |
| HTA72_RS00290 (KPE71T_00310) | 34571..34966 | - | 396 | WP_019725163 | hypothetical protein | - |
| HTA72_RS00445 | 34965..35528 | + | 564 | WP_020277892 | plasmid mobilization protein MobA | - |
| HTA72_RS00300 (KPE71T_00320) | 35515..37494 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| HTA72_RS00305 (KPE71T_00325) | 37508..38008 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| HTA72_RS00310 (KPE71T_00330) | 38005..38784 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| HTA72_RS00315 (KPE71T_00335) | 38795..39958 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| HTA72_RS00320 (KPE71T_00340) | 39948..40207 | + | 260 | Protein_65 | IcmT/TraK family protein | - |
| HTA72_RS00450 | 40232..42187 | + | 1956 | WP_227626114 | DUF5710 domain-containing protein | - |
| HTA72_RS00325 | 42202..43566 | + | 1365 | WP_227626118 | LPD7 domain-containing protein | - |
| HTA72_RS00330 (KPE71T_00355) | 43609..44145 | + | 537 | WP_004187461 | hypothetical protein | traL |
| HTA72_RS00335 (KPE71T_00360) | 44146..44343 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| HTA72_RS00340 (KPE71T_00365) | 44330..44740 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| HTA72_RS00345 (KPE71T_00370) | 44739..45521 | + | 783 | WP_015058941 | DotI/IcmL family type IV secretion protein | traM |
| HTA72_RS00350 (KPE71T_00375) | 45530..46681 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| HTA72_RS00355 (KPE71T_00380) | 46693..48042 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| HTA72_RS00360 (KPE71T_00385) | 48054..48758 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| HTA72_RS00365 (KPE71T_00390) | 48782..49312 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| HTA72_RS00370 | 49329..49718 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| HTA72_RS00375 | 49764..50156 | + | 393 | WP_227626115 | hypothetical protein | - |
| HTA72_RS00380 (KPE71T_00405) | 50254..53304 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| HTA72_RS00385 (KPE71T_00410) | 53301..54509 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| HTA72_RS00390 (KPE71T_00415) | 54506..55102 | + | 597 | WP_015060003 | hypothetical protein | - |
| HTA72_RS00395 (KPE71T_00420) | 55095..57290 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| HTA72_RS00400 (KPE71T_00425) | 57292..57945 | + | 654 | WP_015060005 | hypothetical protein | - |
| HTA72_RS00405 (KPE71T_00430) | 58014..58256 | + | 243 | WP_023893611 | IncL/M type plasmid replication protein RepC | - |
| HTA72_RS00410 (KPE71T_00435) | 58540..59607 | + | 1068 | WP_019725043 | plasmid replication initiator RepA | - |
| HTA72_RS00460 (KPE71T_00440) | 60830..60925 | + | 96 | WP_004206884 | DinQ-like type I toxin DqlB | - |
Host bacterium
| ID | 1013 | GenBank | NC_023027 |
| Plasmid name | pE71T | Incompatibility group | IncL/M |
| Plasmid size | 63578 bp | Coordinate of oriT [Strand] | 35104..35209 [+] |
| Host baterium | Klebsiella pneumoniae |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA8 |