Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100549
Name   oriT_pIMP-PH114 in_silico
Organism   Klebsiella pneumoniae
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_022652 (43773..44323 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pIMP-PH114
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   621 GenBank   YP_008725177
Name   TraI_pIMP-PH114 insolico UniProt ID   G9FJJ6
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>YP_008725177.1 Conjugative transfer protein TraI, relaxase (plasmid) [Klebsiella pneumoniae]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB G9FJJ6


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 41907..67050

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTB75_RS00290 (pCRE114_058) 37938..38171 - 234 WP_001191890 hypothetical protein -
HTB75_RS00295 (pCRE114_059) 38153..38770 - 618 WP_001249395 hypothetical protein -
HTB75_RS00300 (pCRE114_060) 38938..41910 + 2973 WP_001326173 MobH family relaxase -
HTB75_RS00305 (pCRE114_061) 41907..43772 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
HTB75_RS00310 (pCRE114_062) 43783..44367 + 585 WP_000332868 hypothetical protein -
HTB75_RS00315 (pCRE114_063) 44324..44953 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
HTB75_RS00320 (pCRE114_064) 44963..45409 + 447 WP_000122507 hypothetical protein -
HTB75_RS00325 (pCRE114_065) 45419..45796 + 378 WP_000869297 hypothetical protein -
HTB75_RS00330 (pCRE114_066) 45796..46458 + 663 WP_001231464 hypothetical protein -
HTB75_RS00335 (pCRE114_067) 46794..47159 + 366 WP_001052530 hypothetical protein -
HTB75_RS00340 (pCRE114_068) 47304..47585 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
HTB75_RS00345 (pCRE114_069) 47582..48208 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
HTB75_RS00350 (pCRE114_070) 48192..49109 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
HTB75_RS00355 (pCRE114_071) 49109..50422 + 1314 WP_024131605 TraB/VirB10 family protein traB
HTB75_RS00360 (pCRE114_072) 50419..50997 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
HTB75_RS00365 (pCRE114_073) 51001..51393 + 393 WP_000479535 TraA family conjugative transfer protein -
HTB75_RS00370 (pCRE114_074) 51594..57080 + 5487 WP_000606835 Ig-like domain-containing protein -
HTB75_RS00375 (pCRE114_075) 57229..57936 + 708 WP_001259346 DsbC family protein -
HTB75_RS00380 (pCRE114_076) 57933..60380 + 2448 WP_000637384 type IV secretion system protein TraC virb4
HTB75_RS00385 (pCRE114_077) 60395..60712 + 318 WP_000351984 hypothetical protein -
HTB75_RS00390 (pCRE114_078) 60709..61239 + 531 WP_001010740 S26 family signal peptidase -
HTB75_RS00395 (pCRE114_079) 61202..62467 + 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein traW
HTB75_RS00400 (pCRE114_080) 62464..63135 + 672 WP_000575345 EAL domain-containing protein -
HTB75_RS00405 (pCRE114_081) 63135..64139 + 1005 WP_005507569 TraU family protein traU
HTB75_RS00410 (pCRE114_082) 64255..67050 + 2796 WP_001256484 conjugal transfer mating pair stabilization protein TraN traN
HTB75_RS00415 (pCRE114_083) 67089..67949 - 861 WP_023356651 hypothetical protein -
HTB75_RS00420 (pCRE114_084) 68072..68713 - 642 WP_000796664 hypothetical protein -
HTB75_RS00425 (pCRE114_085) 69008..69328 + 321 WP_000547566 hypothetical protein -
HTB75_RS00430 (pCRE114_086) 69632..69817 + 186 WP_001186917 hypothetical protein -
HTB75_RS00435 (pCRE114_087) 70037..71005 + 969 WP_000085160 AAA family ATPase -
HTB75_RS00440 (pCRE114_088) 71016..71924 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   1009 GenBank   NC_022652
Plasmid name   pIMP-PH114 Incompatibility group   IncA/C2
Plasmid size   151885 bp Coordinate of oriT [Strand]   43773..44323 [+]
Host baterium   Klebsiella pneumoniae

Cargo genes

Drug resistance gene   floR, sul2, blaIMP-4, catB3, qacE, sul1, blaDHA-1
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -