Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100547
Name   oriT_p1643_10 in_silico
Organism   Salmonella enterica subsp. enterica serovar Kentucky
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_022522 (49348..49898 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_p1643_10
GAGCAGAGCTATGTGTGACAAGAAGTATAGAGATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   619 GenBank   YP_008605159
Name   TraI_p1643_10 insolico UniProt ID   U3PEX1
Length   992 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>YP_008605159.1 type IV conjugative transport system protein TraI (plasmid) [Salmonella enterica subsp. enterica serovar Kentucky]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB U3PEX1


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 47482..72673

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTB48_RS00355 43507..43740 - 234 WP_001191892 hypothetical protein -
HTB48_RS00360 (p164310_0295) 43722..44339 - 618 WP_001249396 hypothetical protein -
HTB48_RS00365 (p164310_0300) 44507..47485 + 2979 WP_001337757 MobH family relaxase -
HTB48_RS00370 (p164310_0305) 47482..49347 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
HTB48_RS00375 (p164310_0310) 49358..49942 + 585 WP_000332866 hypothetical protein -
HTB48_RS00380 (p164310_0315) 49899..50528 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
HTB48_RS00385 (p164310_0320) 50538..50984 + 447 WP_000122506 hypothetical protein -
HTB48_RS00390 (p164310_0325) 50994..51371 + 378 WP_000869261 hypothetical protein -
HTB48_RS00395 (p164310_0330) 51371..52033 + 663 WP_001231463 hypothetical protein -
HTB48_RS00400 52214..52357 + 144 WP_001275801 hypothetical protein -
HTB48_RS00405 (p164310_0335) 52369..52734 + 366 WP_001052531 hypothetical protein -
HTB48_RS00410 (p164310_0340) 52879..53160 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
HTB48_RS00415 53157..53783 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
HTB48_RS00420 (p164310_0345) 53767..54684 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
HTB48_RS00425 (p164310_0350) 54684..55997 + 1314 WP_024131605 TraB/VirB10 family protein traB
HTB48_RS00430 (p164310_0355) 55994..56572 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
HTB48_RS00435 (p164310_0360) 56576..56968 + 393 WP_000479535 TraA family conjugative transfer protein -
HTB48_RS00440 (p164310_0365) 57169..62700 + 5532 WP_000606833 Ig-like domain-containing protein -
HTB48_RS00445 (p164310_0370) 62849..63556 + 708 WP_001259347 DsbC family protein -
HTB48_RS00450 (p164310_0375) 63553..66000 + 2448 WP_000637386 type IV secretion system protein TraC virb4
HTB48_RS00455 (p164310_0380) 66015..66332 + 318 WP_000351984 hypothetical protein -
HTB48_RS00460 (p164310_0385) 66329..66859 + 531 WP_001010738 S26 family signal peptidase -
HTB48_RS00465 (p164310_0390) 66822..68087 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
HTB48_RS00470 (p164310_0395) 68084..68755 + 672 WP_001337754 EAL domain-containing protein -
HTB48_RS00475 (p164310_0400) 68755..69759 + 1005 WP_043940352 TraU family protein traU
HTB48_RS00480 (p164310_0405) 69875..72673 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
HTB48_RS00485 (p164310_0410) 72712..73572 - 861 WP_000709517 hypothetical protein -
HTB48_RS00490 (p164310_0415) 73695..74336 - 642 WP_000796664 hypothetical protein -
HTB48_RS00495 (p164310_0420) 74630..74950 + 321 WP_000547566 hypothetical protein -
HTB48_RS00500 75254..75439 + 186 WP_001186917 hypothetical protein -
HTB48_RS00505 (p164310_0425) 75659..76627 + 969 WP_000085162 AAA family ATPase -
HTB48_RS00510 (p164310_0430) 76638..77546 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   1007 GenBank   NC_022522
Plasmid name   p1643_10 Incompatibility group   IncA/C2
Plasmid size   167779 bp Coordinate of oriT [Strand]   49348..49898 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Kentucky

Cargo genes

Drug resistance gene   aph(3')-Ia, aph(6)-Id, aph(3'')-Ib, sul2, ant(2'')-Ia, aac(3)-Ia, blaOXA-21, qacE, sul1, blaCTX-M-25
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -