Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100542
• Name: oriT_pCOL-1
• Organism: Pseudomonas aeruginosa
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_022346 (25086..25394 [-], 309 nt)
• oriT length: 309 nt
• IRs (inverted repeats): IR1:79..85, 89..95 (CAAGGGGC..GCCCCTTG) IR2: 153..160, 164..171 (CACGTGGC..GCCATGTG)
• Location of nic site: 190..191
• Conserved sequence flanking the nic site: CATCCTG|T
• Note: predicted by the oriTfinder

oriT sequence

• Length: 309 nt

>oriT_pCOL-1
CGTGTCTCCTTCTCGCCGGGGCGGGGGGCGGGGTGTGTCTTGGACAGTAAGAAGCGAGAGCGCCGCACTGTCCGGGGTCAAGGGGATACCCCTTGCAGGATAAGCCAGACCGTGAGAAGCGAAGCGCCGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGTCATCCTGTATTGCTCATCCGCTTTACTATCGTATCAGCAGTTTAAGTGCACGGGACGCACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAGGAGTTAGCACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_pCOL-1

• ID: 613
• GenBank: YP_008532734
• Name: MobA_pCOL-1
• UniProt ID: T2AC20
• Length: 833 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 833 a.a.
• Molecular weight: 95031.70 Da
• Isoelectric Point: 9.8097

>YP_008532734.1 MobA (plasmid) [Pseudomonas aeruginosa]
MIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINR
VHPETLKVVKINNGFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDME
NRTGEKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKL
QKRLGAYQPPPQRQQVAQREPEPIKPDVPGWKDYITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKAR
RDELMRGNWKGKGEVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQ
WRHRASEPQRIEGASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDST
LAALQLSAQKWGSFTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFE
LYAEAVGAERYRVTSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKK
HHILIDDMNREKLERLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSG
AIHPHRAPGYENRKPKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPA
LELAAASGSAIDAYQRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEG
RDWNDYAQRTARYAYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (12-216)

• RepB DNA-primase from phage plasmid (RepB_primase) (492-750)

• Large polyvalent protein-associated domain 7 (LPD7) (382-470)

Protein structure

• AlphaFold DB: T2AC20

Host bacterium

• ID: 1002
• GenBank: NC_022346
• Plasmid name: pCOL-1
• Incompatibility group: IncP6
• Plasmid size: 31529 bp
• Coordinate of oriT [Strand]: 25086..25394 [-]
• Host baterium: Pseudomonas aeruginosa

Cargo genes

• Drug resistance gene: blaKPC-2
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -