Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100535 |
| Name | oriT_pCFSAN001921  |
| Organism | Salmonella enterica subsp. enterica serovar Typhimurium var. 5- str. CFSAN001921 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_021815 (66467..67017 [+], 551 nt) |
| oriT length | 551 nt |
| IRs (inverted repeats) | IR1: 62..70, 71..79 (AACCCTTTC..GACAGGGTT) IR2: 143..150, 154..161 (TGGCCTGC..GGAGGCCA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 551 nt
>oriT_pCFSAN001921
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 606 | GenBank | WP_001326173 |
| Name | TraI_pCFSAN001921 |
UniProt ID | _ |
| Length | 990 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 990 a.a. Molecular weight: 109356.90 Da Isoelectric Point: 4.9395
>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 64601..89744
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CFSAN001921_RS24060 (CFSAN001921_24105) | 60632..60865 | - | 234 | WP_001191890 | hypothetical protein | - |
| CFSAN001921_RS24065 (CFSAN001921_24110) | 60847..61464 | - | 618 | WP_001249395 | hypothetical protein | - |
| CFSAN001921_RS24070 (CFSAN001921_24115) | 61632..64604 | + | 2973 | WP_001326173 | MobH family relaxase | - |
| CFSAN001921_RS24075 (CFSAN001921_24120) | 64601..66466 | + | 1866 | WP_000178857 | conjugative transfer system coupling protein TraD | virb4 |
| CFSAN001921_RS24080 (CFSAN001921_24125) | 66516..67061 | + | 546 | WP_000228720 | hypothetical protein | - |
| CFSAN001921_RS24085 (CFSAN001921_24130) | 67018..67647 | + | 630 | WP_000743449 | DUF4400 domain-containing protein | tfc7 |
| CFSAN001921_RS24090 (CFSAN001921_24135) | 67657..68103 | + | 447 | WP_000122507 | hypothetical protein | - |
| CFSAN001921_RS24095 (CFSAN001921_24140) | 68113..68490 | + | 378 | WP_000869297 | hypothetical protein | - |
| CFSAN001921_RS24100 (CFSAN001921_24145) | 68490..69152 | + | 663 | WP_001231464 | hypothetical protein | - |
| CFSAN001921_RS24105 (CFSAN001921_24150) | 69488..69853 | + | 366 | WP_001052531 | hypothetical protein | - |
| CFSAN001921_RS24110 (CFSAN001921_24155) | 69998..70279 | + | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| CFSAN001921_RS24115 (CFSAN001921_24160) | 70276..70902 | + | 627 | WP_001049717 | TraE/TraK family type IV conjugative transfer system protein | traE |
| CFSAN001921_RS24120 (CFSAN001921_24165) | 70886..71803 | + | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| CFSAN001921_RS24125 (CFSAN001921_24170) | 71803..73116 | + | 1314 | WP_024131605 | TraB/VirB10 family protein | traB |
| CFSAN001921_RS24130 (CFSAN001921_24175) | 73113..73691 | + | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| CFSAN001921_RS24135 (CFSAN001921_24180) | 73695..74087 | + | 393 | WP_000479535 | TraA family conjugative transfer protein | - |
| CFSAN001921_RS24140 (CFSAN001921_24185) | 74288..79774 | + | 5487 | WP_000606835 | Ig-like domain-containing protein | - |
| CFSAN001921_RS24145 (CFSAN001921_24190) | 79923..80630 | + | 708 | WP_001259346 | DsbC family protein | - |
| CFSAN001921_RS24150 (CFSAN001921_24195) | 80627..83074 | + | 2448 | WP_020833628 | type IV secretion system protein TraC | virb4 |
| CFSAN001921_RS24155 (CFSAN001921_24200) | 83089..83406 | + | 318 | WP_000351984 | hypothetical protein | - |
| CFSAN001921_RS24160 (CFSAN001921_24205) | 83403..83933 | + | 531 | WP_001010740 | S26 family signal peptidase | - |
| CFSAN001921_RS24165 (CFSAN001921_24210) | 83896..85161 | + | 1266 | WP_001447719 | TrbC family F-type conjugative pilus assembly protein | traW |
| CFSAN001921_RS24170 (CFSAN001921_24215) | 85158..85829 | + | 672 | WP_000575345 | EAL domain-containing protein | - |
| CFSAN001921_RS24175 (CFSAN001921_24220) | 85829..86833 | + | 1005 | WP_005507569 | TraU family protein | traU |
| CFSAN001921_RS24180 (CFSAN001921_24225) | 86949..89744 | + | 2796 | WP_020833629 | conjugal transfer mating pair stabilization protein TraN | traN |
| CFSAN001921_RS24185 (CFSAN001921_24230) | 89783..90643 | - | 861 | WP_020833630 | hypothetical protein | - |
| CFSAN001921_RS24190 (CFSAN001921_24235) | 91043..91684 | - | 642 | WP_000134999 | MBL fold metallo-hydrolase | - |
| CFSAN001921_RS24195 (CFSAN001921_24240) | 91924..93186 | - | 1263 | WP_000608644 | IS1380-like element ISEcp1 family transposase | - |
| CFSAN001921_RS24200 (CFSAN001921_24245) | 93465..94115 | - | 651 | WP_020833631 | hypothetical protein | - |
| CFSAN001921_RS24205 (CFSAN001921_24250) | 94409..94729 | + | 321 | WP_000547566 | hypothetical protein | - |
Host bacterium
| ID | 996 | GenBank | NC_021815 |
| Plasmid name | pCFSAN001921 | Incompatibility group | IncA/C2 |
| Plasmid size | 221009 bp | Coordinate of oriT [Strand] | 66467..67017 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Typhimurium var. 5- str. CFSAN001921 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | merR, merT, merP |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |