Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100532
Name   oriT_IncA/C-LS6 in_silico
Organism   Klebsiella pneumoniae
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_021667 (81414..81964 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_IncA/C-LS6
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG

Visualization of oriT structure



Relaxase

ID   603 GenBank   YP_008167050
Name   TraI_IncA/C-LS6 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>YP_008167050.1 TraI (plasmid) [Klebsiella pneumoniae]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 79548..88638

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTA38_RS00505 75579..75812 - 234 WP_001191890 hypothetical protein -
HTA38_RS00510 (D647_p34084) 75794..76411 - 618 WP_001249395 hypothetical protein -
HTA38_RS00515 (D647_p34085) 76579..79551 + 2973 WP_001326173 MobH family relaxase -
HTA38_RS00520 (D647_p34086) 79548..81413 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
HTA38_RS00525 (D647_p34087) 81424..82008 + 585 WP_000332868 hypothetical protein -
HTA38_RS00530 (D647_p34088) 81965..82594 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
HTA38_RS00535 (D647_p34089) 82604..83050 + 447 WP_000122507 hypothetical protein -
HTA38_RS00540 (D647_p34090) 83060..83437 + 378 WP_000869297 hypothetical protein -
HTA38_RS00545 (D647_p34091) 83437..84099 + 663 WP_001231464 hypothetical protein -
HTA38_RS00550 84280..84423 + 144 WP_001275801 hypothetical protein -
HTA38_RS00555 (D647_p34092) 84435..84800 + 366 WP_001052530 hypothetical protein -
HTA38_RS00560 84945..85226 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
HTA38_RS00565 (D647_p34093) 85223..85849 + 627 WP_117082541 TraE/TraK family type IV conjugative transfer system protein traE
HTA38_RS00570 (D647_p34094) 85833..86750 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
HTA38_RS00575 (D647_p34095) 86750..88063 + 1314 WP_024131605 TraB/VirB10 family protein traB
HTA38_RS00580 (D647_p34096) 88060..88638 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
HTA38_RS00585 (D647_p34097) 88642..89034 + 393 WP_000479535 TraA family conjugative transfer protein -
HTA38_RS00590 (D647_p34098) 89319..90581 + 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
HTA38_RS00595 (D647_p34099) 90905..92050 + 1146 WP_014386387 class C beta-lactamase CMY-16 -
HTA38_RS00600 (D647_p34100) 92144..92677 + 534 WP_001221666 lipocalin family protein -
HTA38_RS00605 (D647_p34101) 92674..92991 - 318 WP_000118520 quaternary ammonium compound efflux SMR transporter SugE -
HTA38_RS00610 (D647_p34102) 93248..93613 + 366 Protein_122 LuxR family transcriptional regulator -

Host bacterium

ID   993 GenBank   NC_021667
Plasmid name   IncA/C-LS6 Incompatibility group   IncA/C2
Plasmid size   171925 bp Coordinate of oriT [Strand]   81414..81964 [+]
Host baterium   Klebsiella pneumoniae

Cargo genes

Drug resistance gene   floR, tet(A), aph(6)-Id, aph(3'')-Ib, aph(3')-VIb, sul2, blaCMY-16, dfrA14, ARR-3, cmlA1, blaOXA-10, ant(3'')-Ia, qacE, sul1, qnrA6
Virulence gene   -
Metal resistance gene   merE, merD, merA, merT
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -