Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100525
Name   oriT_pSTU288-2 in_silico
Organism   Salmonella enterica subsp. enterica serovar Typhimurium str. U288
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_021156 (758..917 [-], 160 nt)
oriT length   160 nt
IRs (inverted repeats)      IR1: 3..12, 16..25  (CCAGTTTCTC..GAGAAACCGG)
  IR2: 39..44, 48..53  (CCCTAC..GTAGGG)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TAAGTGCG|CCCT
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 160 nt

>oriT_pSTU288-2
GGCCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCTACAAAGTAGGGTCGGGATTGCCGCCGCTGTGCCTCCATGATAGCCTACGAGACAGCACATTAACAATGGGGTGTCAAGATGGTTAAGGGGAGCAACAAGGCGGCGGATCGGCTGGCCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   596 GenBank   WP_001395566
Name   MobA_pSTU288-2 insolico UniProt ID   C7FHN5
Length   709 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 709 a.a.        Molecular weight: 77951.91 Da        Isoelectric Point: 7.2077

>WP_001395566.1 MULTISPECIES: DNA-primase RepB domain-containing protein [Bacteria]
MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESGHMPEFVERPADYWDAADLYERANGR
LFKEVEFALPVELTLDQQKALASEFAQHLTGAERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQW
FKRYNGKTPEKGGAQKTEALKPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGP
NVVEMEGRGIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTG
RRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGVALEAMANRDA
GFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQM
MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGK
TATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDF
IAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGM
DRGGPDFSM

  Protein domains


Predicted by InterproScan.

RepB DNA-primase from phage plasmid (RepB_primase)

(410-591)

MobA/MobL family (MobA_MobL)

(37-217)


  Protein structure


Source ID Structure
AlphaFold DB C7FHN5


Host bacterium

ID   986 GenBank   NC_021156
Plasmid name   pSTU288-2 Incompatibility group   IncQ1
Plasmid size   11067 bp Coordinate of oriT [Strand]   758..917 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Typhimurium str. U288

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -