Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100518
• Name: oriT_pDPT2
• Organism: Shigella sonnei
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_020251 (5056..5137 [+], 82 nt)
• oriT length: 82 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 82 nt

>oriT_pDPT2
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_pDPT2

• ID: 589
• GenBank: YP_007438843
• Name: MobA_pDPT2
• UniProt ID: L8BU45
• Length: 521 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 521 a.a.
• Molecular weight: 58765.17 Da
• Isoelectric Point: 6.3992

>YP_007438843.1 DNA mobilization protein MobA (plasmid) [Shigella sonnei]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRILTLPDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRDNTERRVQEARKICKQGCDIKRDENQRR
YSPVHSFDRGITEKTPGRGERGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRDDTGEPGRN
QEAERESGEAEREDMGRALSRGEQRTFSGVARGDESRNELDDGKRETECRKAGTGVTEHDGAGKTFAERI
RATATGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDER
LIQEREHALSLERERQLEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

• AlphaFold DB: L8BU45

Host bacterium

• ID: 979
• GenBank: NC_020251
• Plasmid name: pDPT2
• Incompatibility group: ColRNAI
• Plasmid size: 5262 bp
• Coordinate of oriT [Strand]: 5056..5137 [+]
• Host baterium: Shigella sonnei

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -