Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100517
• Name: oriT_pSTE1
• Organism: Staphylococcus hyicus
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_020237 (7868..7908 [+], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTG|TTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 41 nt

>oriT_pSTE1
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_pSTE1

• ID: 588
• GenBank: YP_007419109
• Name: Mob_pSTE1
• UniProt ID: L8BAH8
• Length: 409 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 409 a.a.
• Molecular weight: 48447.75 Da
• Isoelectric Point: 7.0106

>YP_007419109.1 plasmid recombination/mobilization protein (plasmid) [Staphylococcus hyicus]
MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENFINENKTTTRA
VRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRYATVHLDESTPHMHMGIVPFD
KDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIERGQKGSERKNLTVPEFKKLKEEEREIKKELEIKKD
ELMAYTKENKIDKEIDITPIKEMEDVEIETDEKSLFGITKTKTVRQWTGNIVLSEKDYLKLRQKVNKGKQ
AEGKLEAILETDVYQENKELKNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKNQIGDLKEEIKLIY
QSTKRFFKDRISDFKAFKEVFKELADSISNISREKGLDSSFKKEFDRENKKKRTKGMSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: L8BAH8

Host bacterium

• ID: 978
• GenBank: NC_020237
• Plasmid name: pSTE1
• Incompatibility group: _
• Plasmid size: 11951 bp
• Coordinate of oriT [Strand]: 7868..7908 [+]
• Host baterium: Staphylococcus hyicus

Cargo genes

• Drug resistance gene: streptomycin resistance; resistance to macrolides; tetracycline resistance
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -