Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100516
Name   oriT_pEA1509_B in_silico
Organism   Klebsiella aerogenes EA1509E
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_020180 (51151..51701 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pEA1509_B
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   587 GenBank   WP_001337757
Name   TraI_pEA1509_B insolico UniProt ID   _
Length   992 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 49285..74476

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAEROS_RS00420 (ST548_p1086) 45310..45543 - 234 WP_001191892 hypothetical protein -
EAEROS_RS00425 (ST548_p1087) 45525..46142 - 618 WP_001249396 hypothetical protein -
EAEROS_RS00430 (ST548_p1088) 46310..49288 + 2979 WP_001337757 MobH family relaxase -
EAEROS_RS00435 (ST548_p1089) 49285..51150 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
EAEROS_RS00440 (ST548_p1090) 51200..51745 + 546 WP_000228720 hypothetical protein -
EAEROS_RS00445 (ST548_p1091) 51702..52331 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
EAEROS_RS00450 (ST548_p1092) 52341..52787 + 447 WP_000122506 hypothetical protein -
EAEROS_RS00455 (ST548_p1093) 52797..53174 + 378 WP_000869261 hypothetical protein -
EAEROS_RS00460 (ST548_p1094) 53174..53836 + 663 WP_001231463 hypothetical protein -
EAEROS_RS29355 54017..54160 + 144 WP_001275801 hypothetical protein -
EAEROS_RS00465 (ST548_p1097) 54172..54537 + 366 WP_001052531 hypothetical protein -
EAEROS_RS00470 54682..54963 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
EAEROS_RS00475 (ST548_p1099) 54960..55586 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
EAEROS_RS00480 (ST548_p1100) 55570..56487 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
EAEROS_RS00485 (ST548_p1101) 56487..57800 + 1314 WP_024131605 TraB/VirB10 family protein traB
EAEROS_RS00490 (ST548_p1102) 57797..58375 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
EAEROS_RS00495 (ST548_p1103) 58379..58771 + 393 WP_000479535 TraA family conjugative transfer protein -
EAEROS_RS00500 (ST548_p1104) 58972..64503 + 5532 WP_000606833 Ig-like domain-containing protein -
EAEROS_RS00505 (ST548_p1105) 64652..65359 + 708 WP_001259347 DsbC family protein -
EAEROS_RS00510 (ST548_p1106) 65356..67803 + 2448 WP_000637386 type IV secretion system protein TraC virb4
EAEROS_RS00515 (ST548_p1107) 67818..68135 + 318 WP_000351984 hypothetical protein -
EAEROS_RS00520 (ST548_p1108) 68132..68662 + 531 WP_001010738 S26 family signal peptidase -
EAEROS_RS00525 (ST548_p1109) 68625..69890 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
EAEROS_RS00530 (ST548_p1110) 69887..70558 + 672 WP_001337754 EAL domain-containing protein -
EAEROS_RS00535 (ST548_p1111) 70558..71562 + 1005 WP_043940352 TraU family protein traU
EAEROS_RS00540 (ST548_p1112) 71678..74476 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
EAEROS_RS00545 (ST548_p1113) 74515..75375 - 861 WP_000709517 hypothetical protein -
EAEROS_RS00550 (ST548_p1114) 75498..76139 - 642 WP_000796664 hypothetical protein -
EAEROS_RS00555 76433..76753 + 321 WP_000547566 hypothetical protein -
EAEROS_RS00560 (ST548_p1116) 77057..77242 + 186 WP_001186917 hypothetical protein -
EAEROS_RS00565 (ST548_p1118) 77462..78430 + 969 WP_000085162 AAA family ATPase -
EAEROS_RS00570 (ST548_p1119) 78441..79349 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   977 GenBank   NC_020180
Plasmid name   pEA1509_B Incompatibility group   IncA/C2
Plasmid size   162202 bp Coordinate of oriT [Strand]   51151..51701 [+]
Host baterium   Klebsiella aerogenes EA1509E

Cargo genes

Drug resistance gene   sul2, blaTEM-24, dfrA1, ant(3'')-Ia, qacE, sul1
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -