Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100512 |
| Name | oriT_pE66An  |
| Organism | Escherichia coli |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_020086 (20469..21095 [+], 627 nt) |
| oriT length | 627 nt |
| IRs (inverted repeats) | IR1: 198..207, 211..220 (ATTGGGTGTT..AATACCTAAT) IR2:319..324, 330..335 (AAAAAA..TTTTTT) IR3: 393..402, 405..414 (GTATTCATGC..GCATGAATAC) |
| Location of nic site | 301..302 |
| Conserved sequence flanking the nic site |
GGTGT|ATAGC |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 627 nt
>oriT_pE66An
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 583 | GenBank | YP_007349419 |
| Name | TraI_pE66An |
UniProt ID | _ |
| Length | 1078 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 1078 a.a. Molecular weight: 120141.30 Da Isoelectric Point: 6.5229
>YP_007349419.1 conjugal nickase and helicase, similar to traI of plasmid F (plasmid) [Escherichia coli]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43047..53372
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HXG49_RS00240 (D616_p98090) | 38892..40325 | - | 1434 | WP_001288432 | DNA cytosine methyltransferase | - |
| HXG49_RS00245 (D616_p98077) | 40424..40696 | + | 273 | Protein_48 | IS1 family transposase | - |
| HXG49_RS00250 | 40707..40913 | - | 207 | WP_001749967 | hypothetical protein | - |
| HXG49_RS00255 (D616_p98065) | 40918..41565 | - | 648 | WP_015344958 | restriction endonuclease | - |
| HXG49_RS00260 (D616_p98092) | 41615..41848 | - | 234 | WP_001191790 | hypothetical protein | - |
| HXG49_RS00265 (D616_p98068) | 41962..42276 | - | 315 | WP_001749965 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HXG49_RS00270 (D616_p98093) | 42273..42617 | - | 345 | WP_001749964 | hypothetical protein | - |
| HXG49_RS00275 (D616_p98069) | 42633..43004 | - | 372 | WP_011867773 | H-NS family nucleoid-associated regulatory protein | - |
| HXG49_RS00280 (D616_p98034) | 43047..43781 | + | 735 | WP_001749963 | lytic transglycosylase domain-containing protein | virB1 |
| HXG49_RS00285 (D616_p98070) | 43790..44071 | + | 282 | WP_000440698 | transcriptional repressor KorA | - |
| HXG49_RS00290 (D616_p98033) | 44081..44374 | + | 294 | WP_001749962 | hypothetical protein | virB2 |
| HXG49_RS00295 (D616_p98045) | 44424..44741 | + | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
| HXG49_RS00300 (D616_p98044) | 44741..47341 | + | 2601 | WP_001749961 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| HXG49_RS00305 (D616_p98043) | 47359..48072 | + | 714 | WP_001749960 | type IV secretion system protein | virB5 |
| HXG49_RS00310 (D616_p98075) | 48080..48307 | + | 228 | WP_001749959 | IncN-type entry exclusion lipoprotein EexN | - |
| HXG49_RS00315 (D616_p98042) | 48323..49363 | + | 1041 | WP_001749958 | type IV secretion system protein | virB6 |
| HXG49_RS00320 (D616_p98032) | 49434..49592 | + | 159 | WP_012561180 | hypothetical protein | - |
| HXG49_RS00325 (D616_p98041) | 49582..50280 | + | 699 | WP_000646594 | type IV secretion system protein | virB8 |
| HXG49_RS00330 (D616_p98031) | 50291..51175 | + | 885 | WP_000735066 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HXG49_RS00335 (D616_p98040) | 51175..52335 | + | 1161 | WP_000101710 | type IV secretion system protein VirB10 | virB10 |
| HXG49_RS00340 (D616_p98039) | 52377..53372 | + | 996 | WP_000128596 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HXG49_RS00345 (D616_p98064) | 53372..53905 | + | 534 | WP_000792636 | phospholipase D family protein | - |
| HXG49_RS00350 | 54079..54207 | - | 129 | WP_002210551 | DUF6710 family protein | - |
| HXG49_RS00360 (D616_p98094) | 54401..55792 | - | 1392 | WP_001493761 | ISKra4-like element ISKpn19 family transposase | - |
| HXG49_RS00365 (D616_p98030) | 55829..56401 | - | 573 | WP_001493762 | recombinase family protein | - |
| HXG49_RS00370 (D616_p98095) | 56538..57083 | - | 546 | WP_001493763 | plasmid pRiA4b ORF-3 family protein | - |
| HXG49_RS00375 (D616_p98082) | 57221..57430 | - | 210 | Protein_73 | aminoglycoside O-phosphotransferase APH(3'')-Ib | - |
| HXG49_RS00380 (D616_p98055) | 57491..58306 | - | 816 | WP_001043260 | sulfonamide-resistant dihydropteroate synthase Sul2 | - |
Host bacterium
| ID | 973 | GenBank | NC_020086 |
| Plasmid name | pE66An | Incompatibility group | _ |
| Plasmid size | 80105 bp | Coordinate of oriT [Strand] | 20469..21095 [+] |
| Host baterium | Escherichia coli |
Cargo genes
| Drug resistance gene | aac(3)-IId, dfrA14, sul2, tet(D), qnrS1, blaLAP-2, blaCTX-M-14 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |