Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100511
Name   oriT_pB1019 in_silico
Organism   Klebsiella pneumoniae
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019988 (3046..3222 [-], 177 nt)
oriT length   177 nt
IRs (inverted repeats)      5..12, 16..23  (TTCGGGGC..GCCCTGAA)
Location of nic site      56..57
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 177 nt

>oriT_pB1019
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAGTATGTTACGGCGAGATGGAGATTCGGGGAAGTGCTTCATGTGGCAAGGGGGAAAATACGGCTGCGGGGCGTAAGTGCAAAATGCAGGTAACGATGAAATGACGCCTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   582 GenBank   YP_007316681
Name   MbeA_pB1019 insolico UniProt ID   K9MQ37
Length   525 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 525 a.a.        Molecular weight: 57532.66 Da        Isoelectric Point: 5.7934

>YP_007316681.1 MbeA (plasmid) [Klebsiella pneumoniae]
MPVWNGCGMPYWKKGRTMIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELIDASPYVKKY
TSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGKRLQPY
YDRADRPRIDAWQTVVNGRLGLHDPNDPENRRALVTPSGLPETKQEAAETITRGLLMLASSGELKTRDDV
TGALTAAGFEVVRTTKNSISIADPDGGRNIRLRGAIYEQSFNGLEGLRGEIESTAAAYRREAESRIQQAR
ERCQIGTERKREENQRRHQRPRTGDERGDAVEPAERAANGRADVADHHHGVRAAVRQPERGAVVPGEPDS
GEPGGNQAAERYAVETAHADVGRDLSGEPQRTVSGAAGGDESRNGPDGGQQDAERGEAGTGVEQNDGTGK
TIVERVRAATAGLLEKAGRVGERLRGMAEDVWSYATGERGAERARVGLEQAGAEFERAAAPVIDRLNEIE
QQRYGQTTEKEPEKTADLVPERQRTLSHGDREYGD

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(74-253)


  Protein structure


Source ID Structure
AlphaFold DB K9MQ37


Host bacterium

ID   972 GenBank   NC_019988
Plasmid name   pB1019 Incompatibility group   Col440I
Plasmid size   5225 bp Coordinate of oriT [Strand]   3046..3222 [-]
Host baterium   Klebsiella pneumoniae

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -