Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100510
Name   oriT_pB1023 in_silico
Organism   Enterobacter cloacae
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019986 (238..412 [-], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 175 nt

>oriT_pB1023
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACGGATGAGGATGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCGGCACCGGCGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   581 GenBank   YP_007316673
Name   MbeA_pB1023 insolico UniProt ID   K9MQA8
Length   525 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 525 a.a.        Molecular weight: 57504.61 Da        Isoelectric Point: 5.7934

>YP_007316673.1 MbeA (plasmid) [Enterobacter cloacae]
MPVWNGCGMPYWKKGRTMIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELIDASPYVKKY
TSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGKRLQPY
YDRADRPRIDAWQTVVNGRLGLHDPNDPENRRALVTPSGLPETKQEAAETITRGLLMLASSGELKTRDDV
TGALTAAGFEVVRTTKNSISIADPDGGRNIRLRGAIYEQSFNGLEGLRGEIESTAAAYRREAESRIQQAR
ERCQIGTERKREENQRRHQRPRTGDERGDAVEPAERAANGRADVADHHHGVRAAVRQPERGAVVPGEPDS
GEPGGNQAAERYAVETAHADVGRDLSGEPQRTVSGAAGGDESRNGPDGGQQDAERGEAGTGVEQNDGTGK
TIAERVRAATAGLLEKAGRVGERLRGMAEDVWSYATGERGAERARVGLEQAGAEFERAAAPVIDRLNEIE
QQRYGQTTEKEPEKTADLVPERQRTLSHGDREYGD

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(74-253)


  Protein structure


Source ID Structure
AlphaFold DB K9MQA8


Host bacterium

ID   971 GenBank   NC_019986
Plasmid name   pB1023 Incompatibility group   Col440II
Plasmid size   3510 bp Coordinate of oriT [Strand]   238..412 [-]
Host baterium   Enterobacter cloacae

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -