Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100506
Name   oriT_pCIS8 in_silico
Organism   Lactococcus cremoris subsp. cremoris UC509.9
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019430 (71532..71995 [-], 464 nt)
oriT length   464 nt
IRs (inverted repeats)      IR1: 139..148, 153..162  (CCCATTTTAT..ATAAAATGGG)
  IR2: 208..220, 224..236  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 242..256, 269..283  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site      291..292
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 464 nt

>oriT_pCIS8
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATTTATGGATCAGAAAAATGTATCACAAAATCAAACAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   577 GenBank   WP_015081763
Name   MobA_pCIS8 insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47655.35 Da        Isoelectric Point: 8.1230

>WP_015081763.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELEKR
TKQNEFSAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQLAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   967 GenBank   NC_019430
Plasmid name   pCIS8 Incompatibility group   _
Plasmid size   80592 bp Coordinate of oriT [Strand]   71532..71995 [-]
Host baterium   Lactococcus cremoris subsp. cremoris UC509.9

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -