Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100505
Name   oriT_pFOS-HK151325 in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019424 (32471..33020 [-], 550 nt)
oriT length   550 nt
IRs (inverted repeats)      243..255, 257..269  (TATTTAATAATTC..GAATTATTAAATA)
 311..318, 321..328  (GCAAAAAC..GTTTTTGC)
Location of nic site      337..338
Conserved sequence flanking the
  nic site    		  GTAGTGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 550 nt

>oriT_pFOS-HK151325
TTACTCTGGCCATAAGATAAAACCTTTCATTATTAAGCAATGAACTTTTCACTATAAATATGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTATAAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAATTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTCTCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAAACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCGAAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGAGAGCGATATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   576 GenBank   YP_006990792
Name   TraI_pFOS-HK151325 insolico UniProt ID   K7SE05
Length   1756 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191683.22 Da        Isoelectric Point: 5.6912

>YP_006990792.1 conjugal transfer DNA-nicking and unwinding protein TraI (plasmid) [Escherichia coli]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVTLSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQIERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(10-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

AAA domain (AAA_30)

(968-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

DNA helicase TraI (TraI_C)

(1434-1557)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 32083..60217

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTA76_RS00200 (D616_p94054) 28055..28489 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
HTA76_RS00205 (D616_p94055) 28486..29248 + 763 Protein_41 plasmid SOS inhibition protein A -
HTA76_RS00465 29427..29576 + 150 Protein_42 plasmid maintenance protein Mok -
HTA76_RS00210 (D616_p94057) 29518..29643 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
HTA76_RS00215 29962..30258 - 297 Protein_44 hypothetical protein -
HTA76_RS00220 (D616_p94058) 30558..30854 + 297 WP_001272251 hypothetical protein -
HTA76_RS00225 (D616_p94041) 30965..31786 + 822 WP_001234445 DUF932 domain-containing protein -
HTA76_RS00230 (D616_p94059) 32083..32685 - 603 WP_000243713 transglycosylase SLT domain-containing protein virB1
HTA76_RS00235 (D616_p94060) 33008..33391 + 384 WP_001354030 conjugal transfer relaxosome DNA-binding protein TraM -
HTA76_RS00240 (D616_p94061) 33585..34256 + 672 WP_000283561 conjugal transfer transcriptional regulator TraJ -
HTA76_RS00245 (D616_p94062) 34393..34620 + 228 WP_000089263 conjugal transfer relaxosome protein TraY -
HTA76_RS00250 (D616_p94063) 34653..35012 + 360 WP_001098992 type IV conjugative transfer system pilin TraA -
HTA76_RS00255 (D616_p94064) 35027..35338 + 312 WP_000012113 type IV conjugative transfer system protein TraL traL
HTA76_RS00260 (D616_p94065) 35360..35926 + 567 WP_000399780 type IV conjugative transfer system protein TraE traE
HTA76_RS00265 (D616_p94066) 35913..36641 + 729 WP_001230772 type-F conjugative transfer system secretin TraK traK
HTA76_RS00270 (D616_p94067) 36641..38092 + 1452 WP_000146675 F-type conjugal transfer pilus assembly protein TraB traB
HTA76_RS00275 (D616_p94068) 38082..38648 + 567 WP_000896599 conjugal transfer pilus-stabilizing protein TraP -
HTA76_RS00280 (D616_p94069) 38635..38955 + 321 WP_001057307 conjugal transfer protein TrbD -
HTA76_RS00285 (D616_p94070) 38952..39467 + 516 WP_000809881 type IV conjugative transfer system lipoprotein TraV traV
HTA76_RS00290 (D616_p94071) 39602..39823 + 222 WP_001278683 conjugal transfer protein TraR -
HTA76_RS00295 (D616_p94072) 39816..40292 + 477 WP_000549587 hypothetical protein -
HTA76_RS00300 (D616_p94073) 40372..40590 + 219 WP_000556745 hypothetical protein -
HTA76_RS00305 (D616_p94074) 40618..40965 + 348 WP_000836682 hypothetical protein -
HTA76_RS00310 (D616_p94075) 41091..43721 + 2631 WP_015075088 type IV secretion system protein TraC virb4
HTA76_RS00315 (D616_p94076) 43718..44104 + 387 WP_000214084 type-F conjugative transfer system protein TrbI -
HTA76_RS00320 (D616_p94077) 44101..44733 + 633 WP_127471790 type-F conjugative transfer system protein TraW traW
HTA76_RS00325 (D616_p94078) 44730..45722 + 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
HTA76_RS00330 (D616_p94079) 45752..46057 + 306 WP_000224416 hypothetical protein -
HTA76_RS00335 (D616_p94081) 46066..46704 + 639 WP_001080256 type-F conjugative transfer system pilin assembly protein TrbC trbC
HTA76_RS00340 (D616_p94082) 46701..48551 + 1851 WP_000821856 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HTA76_RS00345 (D616_p94083) 48578..48835 + 258 WP_000864353 conjugal transfer protein TrbE -
HTA76_RS00350 (D616_p94084) 48828..49571 + 744 WP_001030371 type-F conjugative transfer system pilin assembly protein TraF traF
HTA76_RS00355 (D616_p94085) 49585..49929 + 345 WP_000556796 conjugal transfer protein TrbA -
HTA76_RS00360 (D616_p94086) 50048..50332 + 285 WP_000624194 type-F conjugative transfer system pilin chaperone TraQ -
HTA76_RS00365 (D616_p94087) 50319..50864 + 546 WP_000059831 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HTA76_RS00370 (D616_p94088) 50794..51141 + 348 WP_001309242 P-type conjugative transfer protein TrbJ -
HTA76_RS00375 (D616_p94089) 51122..51514 + 393 WP_000660699 F-type conjugal transfer protein TrbF -
HTA76_RS00380 (D616_p94090) 51501..52874 + 1374 WP_000944331 conjugal transfer pilus assembly protein TraH traH
HTA76_RS00385 (D616_p94091) 52871..55693 + 2823 WP_001007039 conjugal transfer mating-pair stabilization protein TraG traG
HTA76_RS00390 (D616_p94092) 55709..56194 + 486 WP_000605870 hypothetical protein -
HTA76_RS00395 (D616_p94093) 56243..56974 + 732 WP_000782451 conjugal transfer complement resistance protein TraT -
HTA76_RS00400 (D616_p94094) 57177..57914 + 738 WP_000199905 hypothetical protein -
HTA76_RS00405 (D616_p94095) 57965..60217 + 2253 WP_015059147 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   966 GenBank   NC_019424
Plasmid name   pFOS-HK151325 Incompatibility group   IncFII
Plasmid size   69768 bp Coordinate of oriT [Strand]   32471..33020 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   fosA3, blaTEM-1B, blaCTX-M-3
Virulence gene   -
Metal resistance gene   cadmium resistance
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -