Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100504 |
| Name | oriT_pR148  |
| Organism | Aeromonas hydrophila |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_019380 (47479..48029 [+], 551 nt) |
| oriT length | 551 nt |
| IRs (inverted repeats) | IR1: 62..70, 71..79 (AACCCTTTC..GACAGGGTT) IR2: 143..150, 154..161 (TGGCCTGC..GGAGGCCA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 551 nt
>oriT_pR148
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 575 | GenBank | YP_006965978 |
| Name | PutativerelaxaseofpR148 |
UniProt ID | K4NCG1 |
| Length | 990 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 990 a.a. Molecular weight: 109356.90 Da Isoelectric Point: 4.9395
>YP_006965978.1 type IV conjugative transfer system protein (plasmid) [Aeromonas hydrophila]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | K4NCG1 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 45613..70756
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTB28_RS00335 (D745_p1061) | 41644..41877 | - | 234 | WP_001191890 | hypothetical protein | - |
| HTB28_RS00340 (D745_p1062) | 41859..42476 | - | 618 | WP_001249395 | hypothetical protein | - |
| HTB28_RS00345 (D745_p1046) | 42644..45616 | + | 2973 | WP_001326173 | MobH family relaxase | - |
| HTB28_RS00350 (D745_p1045) | 45613..47478 | + | 1866 | WP_000178857 | conjugative transfer system coupling protein TraD | virb4 |
| HTB28_RS00355 (D745_p1066) | 47489..48073 | + | 585 | WP_000332868 | hypothetical protein | - |
| HTB28_RS00360 (D745_p1069) | 48030..48659 | + | 630 | WP_000743449 | DUF4400 domain-containing protein | tfc7 |
| HTB28_RS00365 (D745_p1064) | 48669..49115 | + | 447 | WP_000122507 | hypothetical protein | - |
| HTB28_RS00370 (D745_p1063) | 49125..49502 | + | 378 | WP_000869297 | hypothetical protein | - |
| HTB28_RS00375 (D745_p1072) | 49502..50164 | + | 663 | WP_001231464 | hypothetical protein | - |
| HTB28_RS00380 (D745_p1100) | 50345..50488 | + | 144 | WP_001275801 | hypothetical protein | - |
| HTB28_RS00385 (D745_p1099) | 50500..50865 | + | 366 | WP_001052530 | hypothetical protein | - |
| HTB28_RS00390 (D745_p1058) | 51010..51291 | + | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| HTB28_RS00395 (D745_p1054) | 51288..51914 | + | 627 | WP_001049717 | TraE/TraK family type IV conjugative transfer system protein | traE |
| HTB28_RS00400 (D745_p1048) | 51898..52815 | + | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| HTB28_RS00405 (D745_p1060) | 52815..54128 | + | 1314 | WP_024131605 | TraB/VirB10 family protein | traB |
| HTB28_RS00410 (D745_p1057) | 54125..54703 | + | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| HTB28_RS00415 (D745_p1059) | 54707..55099 | + | 393 | WP_000479535 | TraA family conjugative transfer protein | - |
| HTB28_RS00420 (D745_p1101) | 55300..60786 | + | 5487 | WP_000606835 | Ig-like domain-containing protein | - |
| HTB28_RS00425 | 60935..61642 | + | 708 | WP_001259346 | DsbC family protein | - |
| HTB28_RS00430 (D745_p1053) | 61639..64086 | + | 2448 | WP_000637384 | type IV secretion system protein TraC | virb4 |
| HTB28_RS00435 (D745_p1068) | 64101..64418 | + | 318 | WP_000351984 | hypothetical protein | - |
| HTB28_RS00440 (D745_p1051) | 64415..64945 | + | 531 | WP_001010740 | S26 family signal peptidase | - |
| HTB28_RS00445 (D745_p1052) | 64908..66173 | + | 1266 | WP_001447719 | TrbC family F-type conjugative pilus assembly protein | traW |
| HTB28_RS00450 (D745_p1067) | 66170..66841 | + | 672 | WP_000575345 | EAL domain-containing protein | - |
| HTB28_RS00455 (D745_p1050) | 66841..67845 | + | 1005 | WP_005507569 | TraU family protein | traU |
| HTB28_RS00460 (D745_p1049) | 67949..70756 | + | 2808 | WP_001447718 | conjugal transfer mating pair stabilization protein TraN | traN |
| HTB28_RS00465 (D745_p1102) | 70795..71655 | - | 861 | WP_000709517 | hypothetical protein | - |
| HTB28_RS00470 | 71778..72419 | - | 642 | WP_000796664 | hypothetical protein | - |
| HTB28_RS00475 | 72714..73034 | + | 321 | WP_000547566 | hypothetical protein | - |
| HTB28_RS00480 (D745_p1105) | 73338..73523 | + | 186 | WP_001186917 | hypothetical protein | - |
| HTB28_RS00485 (D745_p1106) | 73743..74711 | + | 969 | WP_000085160 | AAA family ATPase | - |
| HTB28_RS00490 (D745_p1107) | 74722..75630 | + | 909 | WP_000739139 | hypothetical protein | - |
Host bacterium
| ID | 965 | GenBank | NC_019380 |
| Plasmid name | pR148 | Incompatibility group | IncA/C2 |
| Plasmid size | 165906 bp | Coordinate of oriT [Strand] | 47479..48029 [+] |
| Host baterium | Aeromonas hydrophila |
Cargo genes
| Drug resistance gene | beta-lactamase resistance; streptomycin/spectinomycin resistance; sulfonamide resistance; sulfonamide resistance; tetracycline resistance |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |