Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100500
• Name: oriT_pSB13
• Organism: Shigella boydii
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_019357 (2556..2638 [+], 83 nt)
• oriT length: 83 nt
• IRs (inverted repeats): 3..11, 17..25 (GTGTCGGGG..CCCTGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTGA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 83 nt

>oriT_pSB13
GGGTGTCGGGGTGAAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTGAACCATGCGGCATCAGTGCGGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_pSB13

• ID: 571
• GenBank: YP_006964840
• Name: MobA_pSB13
• UniProt ID: I6M980
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57827.11 Da
• Isoelectric Point: 6.1401

>YP_006964840.1 MobA (plasmid) [Shigella boydii]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELETGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEVGLEVVRVTRS
SISIADPNGGRNIRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQQARKICKQGCDLKRDENQRR
YSPVHSFDRGITEKTPGRGERGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWREDTGEPGRN
QDTGRNIAETEREDMGRGVCAGREKEISGHPAGEIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERLRATA
TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDERLIQE
REHELSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

• AlphaFold DB: I6M980

Host bacterium

• ID: 961
• GenBank: NC_019357
• Plasmid name: pSB13
• Incompatibility group: ColRNAI
• Plasmid size: 5424 bp
• Coordinate of oriT [Strand]: 2556..2638 [+]
• Host baterium: Shigella boydii

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -