Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100499
Name   oriT_pNE1280 in_silico
Organism   Enterobacter cloacae
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019346 (31539..31754 [-], 216 nt)
oriT length   216 nt
IRs (inverted repeats)      46..52, 58..64  (TTCGGGG..CCCTGAA)
Location of nic site      97..98
Conserved sequence flanking the
  nic site    		  ATGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 216 nt

>oriT_pNE1280
GATGGCATGGGATGATATCCAGTAGCTGAGAGGTTGTTAGCGGGTTTCGGGGCGCAACCCTGAACCAGTCACGTAGCGCTAGCAGAGTGTATAATGGCTTATTATGTTGGCATGAGTGCGATAATCAGGAAAGCGCCTCATGTGGCAGGAGAAAAATGCTGCAGCAGAGCGTAAGCAGAATATGCGATACAGAAGATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   570 GenBank   YP_006964655
Name   MobA_pNE1280 insolico UniProt ID   I6TGP3
Length   659 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75087.84 Da        Isoelectric Point: 10.0285

>YP_006964655.1 protein MobA (plasmid) [Enterobacter cloacae]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB I6TGP3


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2903..21874

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTA13_RS00005 (KPC4_0001) 109..426 + 318 WP_004206923 plasmid mobilization protein MobA -
HTA13_RS00010 (KPC4_0003) 413..2392 + 1980 WP_004206924 TraI/MobA(P) family conjugative relaxase -
HTA13_RS00015 (KPC4_0005) 2406..2906 + 501 WP_004206925 DotD/TraH family lipoprotein -
HTA13_RS00020 (KPC4_0007) 2903..3682 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
HTA13_RS00025 (KPC4_0009) 3693..4856 + 1164 WP_004206926 plasmid transfer ATPase TraJ virB11
HTA13_RS00030 4846..5106 + 261 WP_004187310 IcmT/TraK family protein traK
HTA13_RS00420 (KPC4_0011) 5131..8211 + 3081 WP_015062841 LPD7 domain-containing protein -
HTA13_RS00040 8135..8689 + 555 WP_011154467 hypothetical protein traL
HTA13_RS00045 8690..8887 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
HTA13_RS00050 8874..9284 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
HTA13_RS00055 (KPC4_0015) 9283..10065 + 783 WP_015062842 DotI/IcmL family type IV secretion protein traM
HTA13_RS00060 (KPC4_0017) 10074..11225 + 1152 WP_015062843 DotH/IcmK family type IV secretion protein traN
HTA13_RS00065 (KPC4_0019) 11237..12586 + 1350 WP_004206932 conjugal transfer protein TraO traO
HTA13_RS00070 (KPC4_0021) 12598..13302 + 705 WP_004206933 conjugal transfer protein TraP traP
HTA13_RS00075 (KPC4_0023) 13326..13856 + 531 WP_004187478 conjugal transfer protein TraQ traQ
HTA13_RS00080 (KPC4_0025) 13873..14262 + 390 WP_011154472 DUF6750 family protein traR
HTA13_RS00085 14308..14802 + 495 WP_004187480 hypothetical protein -
HTA13_RS00090 (KPC4_0027) 14799..17849 + 3051 WP_015062844 protein TraU traU
HTA13_RS00095 (KPC4_0029) 17846..19054 + 1209 WP_004187486 conjugal transfer protein TraW traW
HTA13_RS00100 19051..19701 + 651 WP_004187488 hypothetical protein -
HTA13_RS00105 (KPC4_0031) 19694..21874 + 2181 WP_004187492 DotA/TraY family protein traY
HTA13_RS00110 (KPC4_0033) 21877..22530 + 654 WP_004206935 hypothetical protein -
HTA13_RS00115 (KPC4_0035) 22604..22834 + 231 WP_004187496 IncL/M type plasmid replication protein RepC -
HTA13_RS00120 (KPC4_0037) 23119..24186 + 1068 WP_020316718 plasmid replication initiator RepA -
HTA13_RS00125 (KPC4_0039) 25352..25885 - 534 Protein_24 IS110-like element IS4321 family transposase -

Host bacterium

ID   960 GenBank   NC_019346
Plasmid name   pNE1280 Incompatibility group   IncL/M
Plasmid size   66531 bp Coordinate of oriT [Strand]   31539..31754 [-]
Host baterium   Enterobacter cloacae

Cargo genes

Drug resistance gene   blaKPC-4
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -