Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100491
• Name: oriT_pUR3036
• Organism: Staphylococcus epidermidis
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_019303 (4..207 [-], 204 nt)
• oriT length: 204 nt
• IRs (inverted repeats): IR1: 51..58, 60..67 (TTGGGGAT..ATCCCCAA) IR2: 93..97, 103..107 (TGGCT..AGCCA)
• Location of nic site: 114..115
• Conserved sequence flanking the nic site: TGCTTG|CCA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 204 nt

>oriT_pUR3036
TGGCTAGCAATTAGCCACTCTTTTTTGCGTAAGCAAAAAACATAAGGGGCTTGGGGATAATCCCCAACAAGCTGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAACCACTTAACGACTTGATAAATTTTGCTTTATCTTATAAAGTAAAATTTAAGAAAGGCGTTATCTTTCGTAGAAAGCGTGGGGGGA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_pUR3036

• ID: 562
• GenBank: YP_006962153
• Name: Relaxase_pUR3036
• UniProt ID: I0B6K6
• Length: 336 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 336 a.a.
• Molecular weight: 38414.55 Da
• Isoelectric Point: 9.8063

>YP_006962153.1 relaxase protein (plasmid) [Staphylococcus epidermidis]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKAARELYGKTDGNQGHVIIQSFQPGEVTPE
QCNQLGLALAEKLVPNHQVAVYTHADTNHVHNHIVLNSIDLNTGKKFNNNKQALRDVRAFNDEVCRAQGL
SVPEKDTARLRYTQTEKAIADPNTTSTAQYSWKDDIREAIDQSTATNMEEFQKHLNQHGITIERVTPKSI
TYRHLAENKKVRGRKLGEDYNKGGIQHGFERQIQRRQQQKHKSDTNFQPKRSTSNRHESTERDTGVTQSD
WDQFAQDTNELERRRQAAESARLVDEKARRDREKRVREKPTSRPIINIDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

• AlphaFold DB: I0B6K6

Host bacterium

• ID: 952
• GenBank: NC_019303
• Plasmid name: pUR3036
• Incompatibility group: _
• Plasmid size: 7209 bp
• Coordinate of oriT [Strand]: 4..207 [-]
• Host baterium: Staphylococcus epidermidis

Cargo genes

• Drug resistance gene: vga(A)LC
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -