Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100484
Name   oriT_pS51B in_silico
Organism   Enterobacter cloacae
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019242 (2269..2443 [-], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 175 nt

>oriT_pS51B
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGCAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACGGGTGCGGGAATCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCGGCACCGGCGCGTAAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   555 GenBank   YP_006961018
Name   Mob_pS51B insolico UniProt ID   E9RGW3
Length   512 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 512 a.a.        Molecular weight: 56685.31 Da        Isoelectric Point: 8.5860

>YP_006961018.1 mobilization protein A (plasmid) [Enterobacter cloacae]
MPDLSGCGTPFLRKERTMIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVRELIDASPYVKKY
TSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGRRLQPY
YDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSGLPKTKQEAAEAITRGLLTLASSGELKSRQDV
TEALERSGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGLRAAIESAEAEYRRDAESRIQRAR
AVCQSGTERKREENQRRHPRPRAEYERGDGEKPAERTADCRADMAHHPDGVSAADRHEREHSVVAGAADN
RQLHASSSAGRYPGENDRPDVGSALSGEQRRPEVSYPAAGDANRSHPLRRDDMDPPETGVAEDDRAGKAV
AERIRAATAGLLAKAGRMGERLRGMAEHVWAYATGERGAERARVGLEHAGAEFKRAAAPFVRELNAIEEK
AVLEYQRQNELELTLRQKQKSF

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(74-255)


  Protein structure


Source ID Structure
AlphaFold DB E9RGW3


Host bacterium

ID   945 GenBank   NC_019242
Plasmid name   pS51B Incompatibility group   ColRNAI
Plasmid size   4854 bp Coordinate of oriT [Strand]   2269..2443 [-]
Host baterium   Enterobacter cloacae

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -